703483

antibody from Invitrogen Antibodies

Targeting: AVPR2 DIR, DIR3, V2R

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

703483

Provider

Invitrogen Antibodies

Product name

AVPR2 Recombinant Rabbit Monoclonal Antibody (7H28L47)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Mouse, Rat, Bovine.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

7H28L47

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells transiently transfected with AVPR2 were grown on poly-L-lysine-coated coverslips overnight. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-AVPR2 Recombinant Rabbit Monoclonal Antibody (Product # 703483, 1:500 dilution). Cells were then incubated with Alexa4 88-conjugated secondary antibody for 2 h at room temperature, mounted and examined using an Immunofluorescence microscope. Note, strong staining of transfected cells.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sections of human kidney were dewaxed, microwaved in citric acid and incubated with Anti-AVPR2 Recombinant Rabbit Monoclonal Antibody (Product # 703483, 1:500 dilution). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, peptide neutralization control.