Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX24755 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX24755, RRID:AB_373262
- Product name
- URM1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Yeast
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of Urm1 fusion protein. Anti- Urm1 antibody generated by immunization with recombinant yeast Urm1 was tested by western blot against yeast lysates expressing the Urm1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of GeneTex's anti-GFP
