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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- ELISA [1]
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- Product number
- H00051428-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00051428-M01, RRID:AB_538214
- Product name
- Mouse Monoclonal DDX41 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DDX41 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 41
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references High-Throughput Screening to Identify Inhibitors of DEAD Box Helicase DDX41.
Structural and Functional Analysis of DDX41: a bispecific immune receptor for DNA and cyclic dinucleotide.
Positive feedback regulation of type I IFN production by the IFN-inducible DNA sensor cGAS.
The helicase DDX41 recognizes the bacterial secondary messengers cyclic di-GMP and cyclic di-AMP to activate a type I interferon immune response.
The helicase DDX41 senses intracellular DNA mediated by the adaptor STING in dendritic cells.
Yoneyama-Hirozane M, Kondo M, Matsumoto SI, Morikawa-Oki A, Morishita D, Nakanishi A, Kawamoto T, Nakayama M
SLAS discovery : advancing life sciences R & D 2017 Oct;22(9):1084-1092
SLAS discovery : advancing life sciences R & D 2017 Oct;22(9):1084-1092
Structural and Functional Analysis of DDX41: a bispecific immune receptor for DNA and cyclic dinucleotide.
Omura H, Oikawa D, Nakane T, Kato M, Ishii R, Ishitani R, Tokunaga F, Nureki O
Scientific reports 2016 Oct 10;6:34756
Scientific reports 2016 Oct 10;6:34756
Positive feedback regulation of type I IFN production by the IFN-inducible DNA sensor cGAS.
Ma F, Li B, Liu SY, Iyer SS, Yu Y, Wu A, Cheng G
Journal of immunology (Baltimore, Md. : 1950) 2015 Feb 15;194(4):1545-54
Journal of immunology (Baltimore, Md. : 1950) 2015 Feb 15;194(4):1545-54
The helicase DDX41 recognizes the bacterial secondary messengers cyclic di-GMP and cyclic di-AMP to activate a type I interferon immune response.
Parvatiyar K, Zhang Z, Teles RM, Ouyang S, Jiang Y, Iyer SS, Zaver SA, Schenk M, Zeng S, Zhong W, Liu ZJ, Modlin RL, Liu YJ, Cheng G
Nature immunology 2012 Dec;13(12):1155-61
Nature immunology 2012 Dec;13(12):1155-61
The helicase DDX41 senses intracellular DNA mediated by the adaptor STING in dendritic cells.
Zhang Z, Yuan B, Bao M, Lu N, Kim T, Liu YJ
Nature immunology 2011 Sep 4;12(10):959-65
Nature immunology 2011 Sep 4;12(10):959-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX41 Antibody (2F4) [H00051428-M01] - Analysis of DDX41 expression in transfected 293T cell line by DDX41 monoclonal antibody (M01), clone 2F4.Lane 1: DDX41 transfected lysate(70 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX41 Antibody (2F4) [H00051428-M01] - DDX41 monoclonal antibody (M01), clone 2F4 Analysis of DDX41 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: DDX41 Antibody (2F4) [H00051428-M01] - Detection limit for recombinant GST tagged DDX41 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: DDX41 Antibody (2F4) [H00051428-M01] - Analysis of monoclonal antibody to DDX41 on HeLa cell. Antibody concentration 10 ug/ml.
