GTX102595

antibody from GeneTex

Targeting: TP53BP1 53BP1, p202, TDRD30

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX102595

Provider

GeneTex

Proper citation

Product name

53BP1 antibody [N1], N-term

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Genome Stability by DNA Polymerase β in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development.

Onishi K, Uyeda A, Shida M, Hirayama T, Yagi T, Yamamoto N, Sugo N
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Aug 30;37(35):8444-8458

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Middle infrared radiation induces G2/M cell cycle arrest in A549 lung cancer cells.

Chang HY, Shih MH, Huang HC, Tsai SR, Juan HF, Lee SC
PloS one 2013;8(1):e54117

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (50 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod no

Proprietary.

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod page

KD Reagent Prod page

Antibody Lot Number

39848

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

53BP1 antibody [N1], N-term detects TP53BP1 protein by Western blot analysis.A. 30 ug 293T whole cell lysate/extractB. 30 whole cell lysate/extract of human 53BP1-transfected 293T cells5 % SDS-PAGE53BP1 antibody [N1], N-term (GTX102595) dilution: 1:5000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

53BP1 antibody [N1], N-term detects TP53BP1 protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g HeLa whole cell lysate/extract5 % SDS-PAGE53BP1 antibody [N1], N-term (GTX102595) dilution: 1:500

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (50 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: 53BP1 protein stained by 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. 53BP1 antibody [N1], N-term (GTX102595) dilution: 1:250.