GTX102425

antibody from GeneTex

Targeting: TP63 EEC3, KET, NBP, OFC8, p51, p53CP, p63, p73H, p73L, SHFM4, TP53CP, TP53L, TP73L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

GTX102425

Provider

GeneTex

Proper citation

GeneTex Cat#GTX102425, RRID:AB_1952344

Product name

p63 antibody [N2C1], Internal

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Corneal Wound Healing Effects of Mesenchymal Stem Cell Secretome Delivered Within a Viscoelastic Gel Carrier.

Fernandes-Cunha GM, Na KS, Putra I, Lee HJ, Hull S, Cheng YC, Blanco IJ, Eslani M, Djalilian AR, Myung D
Stem cells translational medicine 2019 May;8(5):478-489

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TFAP2C- and p63-Dependent Networks Sequentially Rearrange Chromatin Landscapes to Drive Human Epidermal Lineage Commitment.

Li L, Wang Y, Torkelson JL, Shankar G, Pattison JM, Zhen HH, Fang F, Duren Z, Xin J, Gaddam S, Melo SP, Piekos SN, Li J, Liaw EJ, Chen L, Li R, Wernig M, Wong WH, Chang HY, Oro AE
Cell stem cell 2019 Feb 7;24(2):271-284.e8

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Treatment of intractable oral ulceration with an oral mucosa equivalent.

Lee J, Shin D, Roh JL
Journal of biomedical materials research. Part B, Applied biomaterials 2018 Nov 12;

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Myoepithelial Cells of Submucosal Glands Can Function as Reserve Stem Cells to Regenerate Airways after Injury.

Tata A, Kobayashi Y, Chow RD, Tran J, Desai A, Massri AJ, McCord TJ, Gunn MD, Tata PR
Cell stem cell 2018 May 3;22(5):668-683.e6

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Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.

Nguyen QH, Pervolarakis N, Blake K, Ma D, Davis RT, James N, Phung AT, Willey E, Kumar R, Jabart E, Driver I, Rock J, Goga A, Khan SA, Lawson DA, Werb Z, Kessenbrock K
Nature communications 2018 May 23;9(1):2028

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Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity.

Tata PR, Chow RD, Saladi SV, Tata A, Konkimalla A, Bara A, Montoro D, Hariri LP, Shih AR, Mino-Kenudson M, Mou H, Kimura S, Ellisen LW, Rajagopal J
Developmental cell 2018 Mar 26;44(6):679-693.e5

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Impact of RUNX2 gene silencing on the gemcitabine sensitivity of p53‑mutated pancreatic cancer MiaPaCa‑2 spheres.

Sang M, Nakamura M, Ogata T, Sun D, Shimozato O, Nikaido T, Ozaki T
Oncology reports 2018 Jun;39(6):2749-2758

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Extracellular galectin-1 enhances adhesion to and invasion of oral epithelial cells by Porphyromonas gingivalis.

Tamai R, Kobayashi-Sakamoto M, Kiyoura Y
Canadian journal of microbiology 2018 Jul;64(7):465-471

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Identification of novel ΔNp63α-regulated miRNAs using an optimized small RNA-Seq analysis pipeline.

Sakaram S, Craig MP, Hill NT, Aljagthmi A, Garrido C, Paliy O, Bottomley M, Raymer M, Kadakia MP
Scientific reports 2018 Jul 3;8(1):10069

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PAX9 regulates squamous cell differentiation and carcinogenesis in the oro-oesophageal epithelium.

Xiong Z, Ren S, Chen H, Liu Y, Huang C, Zhang YL, Odera JO, Chen T, Kist R, Peters H, Garman K, Sun Z, Chen X
The Journal of pathology 2018 Feb;244(2):164-175

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A revised airway epithelial hierarchy includes CFTR-expressing ionocytes.

Montoro DT, Haber AL, Biton M, Vinarsky V, Lin B, Birket SE, Yuan F, Chen S, Leung HM, Villoria J, Rogel N, Burgin G, Tsankov AM, Waghray A, Slyper M, Waldman J, Nguyen L, Dionne D, Rozenblatt-Rosen O, Tata PR, Mou H, Shivaraju M, Bihler H, Mense M, Tearney GJ, Rowe SM, Engelhardt JF, Regev A, Rajagopal J
Nature 2018 Aug;560(7718):319-324

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Polymerase-mediated ultramutagenesis in mice produces diverse cancers with high mutational load.

Li HD, Cuevas I, Zhang M, Lu C, Alam MM, Fu YX, You MJ, Akbay EA, Zhang H, Castrillon DH
The Journal of clinical investigation 2018 Aug 31;128(9):4179-4191

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Human esophageal myofibroblast secretion of bone morphogenetic proteins and GREMLIN1 and paracrine regulation of squamous epithelial growth.

Zhang C, Niu C, Yang K, Shaker A
Scientific reports 2018 Aug 17;8(1):12354

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Screening for long noncoding RNAs associated with oral squamous cell carcinoma reveals the potentially oncogenic actions of DLEU1.

Nishiyama K, Maruyama R, Niinuma T, Kai M, Kitajima H, Toyota M, Hatanaka Y, Igarashi T, Kobayashi JI, Ogi K, Dehari H, Miyazaki A, Yorozu A, Yamamoto E, Idogawa M, Sasaki Y, Sugai T, Tokino T, Hiratsuka H, Suzuki H
Cell death & disease 2018 Aug 1;9(8):826

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p63α protein up-regulates heat shock protein 70 expression via E2F1 transcription factor 1, promoting Wasf3/Wave3/MMP9 signaling and bladder cancer invasion.

Jin H, Xie Q, Guo X, Xu J, Wang A, Li J, Zhu J, Wu XR, Huang H, Huang C
The Journal of biological chemistry 2017 Sep 22;292(38):15952-15963

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Identification of the SOX2 Interactome by BioID Reveals EP300 as a Mediator of SOX2-dependent Squamous Differentiation and Lung Squamous Cell Carcinoma Growth.

Kim BR, Coyaud E, Laurent EMN, St-Germain J, Van de Laar E, Tsao MS, Raught B, Moghal N
Molecular & cellular proteomics : MCP 2017 Oct;16(10):1864-1888

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Myosin X is recruited to nascent focal adhesions at the leading edge and induces multi-cycle filopodial elongation.

He K, Sakai T, Tsukasaki Y, Watanabe TM, Ikebe M
Scientific reports 2017 Oct 20;7(1):13685

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Fibroblast Growth Factor Receptor 2 (FGFR2) Is Required for Meibomian Gland Homeostasis in the Adult Mouse.

Reneker LW, Wang L, Irlmeier RT, Huang AJW
Investigative ophthalmology & visual science 2017 May 1;58(5):2638-2646

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Identification and characterization of the intercellular adhesion molecule-2 gene as a novel p53 target.

Sasaki Y, Tamura M, Takeda K, Ogi K, Nakagaki T, Koyama R, Idogawa M, Hiratsuka H, Tokino T
Oncotarget 2016 Sep 20;7(38):61426-61437

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A mucosal imprint left by prior Escherichia coli bladder infection sensitizes to recurrent disease.

O'Brien VP, Hannan TJ, Yu L, Livny J, Roberson ED, Schwartz DJ, Souza S, Mendelsohn CL, Colonna M, Lewis AL, Hultgren SJ
Nature microbiology 2016 Oct 31;2:16196

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Transcriptome and proteome characterization of surface ectoderm cells differentiated from human iPSCs.

Qu Y, Zhou B, Yang W, Han B, Yu-Rice Y, Gao B, Johnson J, Svendsen CN, Freeman MR, Giuliano AE, Sareen D, Cui X
Scientific reports 2016 Aug 23;6:32007

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Amplification and overexpression of TP63 and MYC as biomarkers for transition of cervical intraepithelial neoplasia to cervical cancer.

Zhu D, Jiang XH, Jiang YH, Ding WC, Zhang CL, Shen H, Wang XL, Ma D, Hu Z, Wang H
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society 2014 May;24(4):643-8

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Simvastatin induced HCT116 colorectal cancer cell apoptosis through p38MAPK-p53-survivin signaling cascade.

Chang HL, Chen CY, Hsu YF, Kuo WS, Ou G, Chiu PT, Huang YH, Hsu MJ
Biochimica et biophysica acta 2013 Aug;1830(8):4053-64

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

p63 antibody [N2C1], Internal detects TP63 protein by western blot analysis.A. 50 ?g mouse brain lysate/extract7.5% SDS-PAGEp63 antibody [N2C1], Internal (GTX102425) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

p63 antibody [N2C1], Internal detects TP63 protein by western blot analysis.A. 50 ?g rat brain lysate/extract7.5% SDS-PAGEp63 antibody [N2C1], Internal (GTX102425) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

p63 antibody detects p63 protein by western blot analysis.A. 30 ?g A431 whole cell lysate/extract 7.5 % SDS-PAGEp63 antibody (GTX102425) dilution: 1:500

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

p63 antibody detects p63 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody (GTX102425) at a dilution of 1:2500.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [N2C1], Internal (GTX102425) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [N2C1], Internal (GTX102425) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

p63 antibody detects p63 protein by Western blot analysis. Whole cell extracts (30 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody (GTX102425) diluted by 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) A431 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [N2C1], Internal (GTX102425) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

p63 antibody [N2C1], Internal detects p63 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p63 protein stained by p63 antibody [N2C1], Internal (GTX102425) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of p63 protein from A431 whole cell extracts using 5 £gg of p63 antibody [N2C1], Internal (GTX102425).Western blot analysis was performed using p63 antibody [N2C1], Internal (GTX102425).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using p63(GTX102425) antibody at 1:100 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical characterization of Synaptophysin (GTX100865), p63 (GTX102425) and Cytokeratin 7 (GTX109723) in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody at 1:500 overnight at 4¢J and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.