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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB0088 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB0088, RRID:AB_849215
- Product name
- TLR3 monoclonal antibody, clone 40C1285.6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic peptide of TLR3.
- Isotype
- IgG
- Antibody clone number
- 40C1285.6
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Human β-defensin-3 correlates with muscle fibre degeneration in idiopathic inflammatory myopathies.
Viral double-stranded RNA sensors induce antiviral, pro-inflammatory, and pro-apoptotic responses in human renal tubular epithelial cells.
SerpinB9 expression in human renal tubular epithelial cells is induced by triggering of the viral dsRNA sensors TLR3, MDA5 and RIG-I.
Double-stranded RNA mediates interferon regulatory factor 3 activation and interleukin-6 production by engaging Toll-like receptor 3 in human brain astrocytes.
Identification of Toll-like receptor 3 as a potential therapeutic target in clear cell renal cell carcinoma.
Key differences in TLR3/poly I:C signaling and cytokine induction by human primary cells: a phenomenon absent from murine cell systems.
Effects of single nucleotide polymorphisms on Toll-like receptor 3 activity and expression in cultured cells.
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Güttsches AK, Jacobsen F, Theiss C, Rittig A, Rehimi R, Kley RA, Vorgerd M, Steinstraesser L
Innate immunity 2014 Jan;20(1):49-60
Innate immunity 2014 Jan;20(1):49-60
Viral double-stranded RNA sensors induce antiviral, pro-inflammatory, and pro-apoptotic responses in human renal tubular epithelial cells.
Heutinck KM, Rowshani AT, Kassies J, Claessen N, van Donselaar-van der Pant KA, Bemelman FJ, Eldering E, van Lier RA, Florquin S, Ten Berge IJ, Hamann J
Kidney international 2012 Sep;82(6):664-75
Kidney international 2012 Sep;82(6):664-75
SerpinB9 expression in human renal tubular epithelial cells is induced by triggering of the viral dsRNA sensors TLR3, MDA5 and RIG-I.
Heutinck KM, Kassies J, Florquin S, ten Berge IJ, Hamann J, Rowshani AT
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2012 Jul;27(7):2746-54
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2012 Jul;27(7):2746-54
Double-stranded RNA mediates interferon regulatory factor 3 activation and interleukin-6 production by engaging Toll-like receptor 3 in human brain astrocytes.
Kim H, Yang E, Lee J, Kim SH, Shin JS, Park JY, Choi SJ, Kim SJ, Choi IH
Immunology 2008 Aug;124(4):480-8
Immunology 2008 Aug;124(4):480-8
Identification of Toll-like receptor 3 as a potential therapeutic target in clear cell renal cell carcinoma.
Morikawa T, Sugiyama A, Kume H, Ota S, Kashima T, Tomita K, Kitamura T, Kodama T, Fukayama M, Aburatani H
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Oct 1;13(19):5703-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Oct 1;13(19):5703-9
Key differences in TLR3/poly I:C signaling and cytokine induction by human primary cells: a phenomenon absent from murine cell systems.
Lundberg AM, Drexler SK, Monaco C, Williams LM, Sacre SM, Feldmann M, Foxwell BM
Blood 2007 Nov 1;110(9):3245-52
Blood 2007 Nov 1;110(9):3245-52
Effects of single nucleotide polymorphisms on Toll-like receptor 3 activity and expression in cultured cells.
Ranjith-Kumar CT, Miller W, Sun J, Xiong J, Santos J, Yarbrough I, Lamb RJ, Mills J, Duffy KE, Hoose S, Cunningham M, Holzenburg A, Mbow ML, Sarisky RT, Kao CC
The Journal of biological chemistry 2007 Jun 15;282(24):17696-705
The Journal of biological chemistry 2007 Jun 15;282(24):17696-705
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20 ug/lane human intestine tissue lysate (lane C). Using TLR3 monoclonal antibody, clone 40C1285.6 (Cat # MAB0088).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- IHC analysis of TLR3 in (A) human gut lumen (longitudinal section, transverse region). Using TLR3 monoclonal antibody, clone 40C1285.6 (Cat # MAB0088) at 10 ug/mL (Data courtesy Dr. Elizabeth Furrie, University of Dundee) and mouse spleen tissue using isotype control (B) and TLR3 monoclonal antibody, clone 40C1285.6 (Cat # MAB0088) (C) at 5 mg/mL .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Intracellular flow analysis of TLR3 in Ramos cells. Using 2 ug of TLR3 monoclonal antibody, clone 40C1285.6 (Cat # MAB0088). Shaded histogram represents Ramos cells without antibody; Green represents isotype control; red represents anti-TLR3 antibody.
- Validation comment
- Flow Cytometry
