Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [3]
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Validation data
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- Product number
- PA5-29619 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLR3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: K562, THP-1.
- Concentration
- 1.4 mg/mL
Submitted references Myeloid Differentiation Primary Response 88-Cyclin D1 Signaling in Breast Cancer Cells Regulates Toll-Like Receptor 3-Mediated Cell Proliferation.
TLR3 absence confers increased survival with improved macrophage activity against pneumonia.
Singh A, Devkar R, Basu A
Frontiers in oncology 2020;10:1780
Frontiers in oncology 2020;10:1780
TLR3 absence confers increased survival with improved macrophage activity against pneumonia.
Suresh MV, Dolgachev VA, Zhang B, Balijepalli S, Swamy S, Mooliyil J, Kralovich G, Thomas B, Machado-Aranda D, Karmakar M, Lalwani S, Subramanian A, Anantharam A, Moore BB, Raghavendran K
JCI insight 2019 Dec 5;4(23)
JCI insight 2019 Dec 5;4(23)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLR3/CD283 using 30 µg of A) K562 and B) THP-1 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a TLR3/CD283 polyclonal antibody (Product # PA5-29619) at a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TLR3/CD283 in methanol-fixed HeLa cells using a TLR3/CD283 polyclonal antibody (Product # PA5-29619) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using TLR3 (Product # PA5-29619) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- TLR3 Polyclonal Antibody detects TLR3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. TLR3 stained by TLR3 Polyclonal Antibody (Product # PA5-29619) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TLR3 Polyclonal Antibody detects TLR3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. TLR3 stained by TLR3 Polyclonal Antibody (Product # PA5-29619) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Effect of MyD88 inhibitor on surface localization of TLR3. Fluorescent microscopy image of cells, treated with TLR3 ligand (10 mug/ml) with or without MyD88 inhibitor (1 muM) following immunocytochemical staining with antibody against TLR3 and Alexa 594-tagged secondary antibody and counterstained with DAPI. (A) T47D cell and (B) MDA-MB-231 cells. (C,D) Bar graph showing the localization of TLR3 in cell surface after observation through a microscope and analyses through the ImageJ package for all the experiment groups. The results are presented as mean +- SD ( p < 0.05 is treated as significant).