NB110-40499

antibody from Novus Biologicals

Targeting: KDM5A JARID1A, RBBP2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB110-40499

Provider

Novus Biologicals

Proper citation

Novus Cat#NB110-40499, RRID:AB_669526

Product name

Rabbit Polyclonal Jarid1A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Identification of ryuvidine as a KDM5A inhibitor.

Mitsui E, Yoshida S, Shinoda Y, Matsumori Y, Tsujii H, Tsuchida M, Wada S, Hasegawa M, Ito A, Mino K, Onuki T, Yoshida M, Sasaki R, Mizukami T
Scientific reports 2019 Jul 9;9(1):9952

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Increased progesterone receptor A expression in labouring human myometrium is associated with decreased promoter occupancy by the histone demethylase JARID1A.

Chai SY, Smith R, Fitter JT, Mitchell C, Pan X, Ilicic M, Maiti K, Zakar T, Madsen G
Molecular human reproduction 2014 May;20(5):442-53

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Histone demethylase KDM5A is an integral part of the core Notch-RBP-J repressor complex.

Liefke R, Oswald F, Alvarado C, Ferres-Marco D, Mittler G, Rodriguez P, Dominguez M, Borggrefe T
Genes & development 2010 Mar 15;24(6):590-601

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Chromatin remodeling: recruitment of histone demethylase RBP2 by Mad1 for transcriptional repression of a Myc target gene, telomerase reverse transcriptase.

Ge Z, Li W, Wang N, Liu C, Zhu Q, Björkholm M, Gruber A, Xu D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Feb;24(2):579-86

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p53-targeted LSD1 functions in repression of chromatin structure and transcription in vivo.

Tsai WW, Nguyen TT, Shi Y, Barton MC
Molecular and cellular biology 2008 Sep;28(17):5139-46

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Jarid1A Antibody [NB110-40499] - Detection of Human JARID1A/RBP2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1A/RBP2 antibody NB110-40499 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Jarid1A Antibody [NB110-40499] - Detection of Human and Mouse Jarid1A/RBP2 on HeLa whole cell lysate using NB110-40499. Jarid1A/RBP2 was also immunoprecipitated by other rabbit anti-Jarid1A/RBP2 antibodies.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Jarid1A Antibody [NB110-40499] - Whole cell lysate (50 ug) from TCMK-1 and Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1A/RBP2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Jarid1A Antibody [NB110-40499] - Detection of human JARID1A/RBP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JARID1A/RBP2 antibody NB110-40499 (lot NB110-40499-3) used for IP at 6 ug per reaction. JARID1A/RBP2 was also immunoprecipitated by a previous lot of this antibody (lot NB110-40499-2). For blotting immunoprecipitated JARID1A/RBP2, NB110-40499 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.