sc-7964

antibody from Santa Cruz Biotechnology

Targeting: TSG101 TSG10, VPS23

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-7964

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-7964, RRID:AB_671392

Product name

Anti-TSG101

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Submitted references

HIV Nef is secreted in exosomes and triggers apoptosis in bystander CD4+ T cells.

Lenassi M, Cagney G, Liao M, Vaupotic T, Bartholomeeusen K, Cheng Y, Krogan NJ, Plemenitas A, Peterlin BM
Traffic (Copenhagen, Denmark) 2010 Jan;11(1):110-22

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Essential role of Hrs in a recycling mechanism mediating functional resensitization of cell signaling.

Hanyaloglu AC, McCullagh E, von Zastrow M
The EMBO journal 2005 Jul 6;24(13):2265-83

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 32

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.