MA5-32687

antibody from Invitrogen Antibodies

Targeting: ABCC2 CMOAT, cMRP, DJS, MRP2

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32687

Provider

Invitrogen Antibodies

Product name

MRP2 Recombinant Rabbit Monoclonal Antibody (JA32-01)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JA32-01

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Generation of Human Pluripotent Stem Cell-Derived Polarized Hepatocytes.

Bushweller L, Zhao Y, Zhang F, Wu X
Current protocols 2022 Jan;2(1):e345

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of MRP2 in HepG2 cells using a MRP2 Monoclonal antibody (Product # MA5-32687) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of MRP2 in 293T cells using a MRP2 Monoclonal antibody (Product # MA5-32687) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of MRP2 of paraffin-embedded Human liver tissue using a MRP2 Monoclonal antibody (Product #MA5-32687). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of MRP2 of paraffin-embedded Human kidney tissue using a MRP2 Monoclonal antibody (Product #MA5-32687). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of MRP2 of paraffin-embedded Human pancreas tissue using a MRP2 Monoclonal antibody (Product #MA5-32687). Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of MRP2 in A549 cells using a MRP2 Monoclonal Antibody (Product # MA5-32687) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of MRP2 in A549 cells using a MRP2 Monoclonal Antibody (Product # MA5-32687) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

4 Figure Distribution of membrane proteins TFRC and MRP2 on pol-HLCs. ( A ) pol-HLCs were incubated with 25 ug/ml of transferrin-488 (green) for 10 min at 37degC prior to washing and staining with anti-ZO-1 (red). Scale bars = 15 mum. Bottom panels: xz images from cross sections indicated by the dashed line in the corresponding xy images above. ( B ) Cross-sectional views ( xz ) of nonpolarized HLCs (non-pol HLCs) and pol-HLCs stained for MRP2 and ZO-1. This figure is adapted from Dao Thi et al. ( 2020).