PA5-96559

antibody from Invitrogen Antibodies

Targeting: EFTUD2 SNRNP116, Snrp116, Snu114, U5-116KD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96559

Provider

Invitrogen Antibodies

Product name

EFTUD2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.49 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of EFTUD2 in extracts of various cell lines using EFTUD2 Polyclonal Antibody (Product # PA5-96559) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of EFTUD2 in extracts of various cell lines using EFTUD2 Polyclonal Antibody (Product # PA5-96559) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of EFTUD2 was performed in C6 cells using EFTUD2 Polyclonal Antibody (Product # PA5-96559).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of EFTUD2 was performed in HeLa cells using EFTUD2 Polyclonal Antibody (Product # PA5-96559) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of EFTUD2 was performed in L929 cells using EFTUD2 Polyclonal Antibody (Product # PA5-96559) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EFTUD2 in paraffin-embedded human gastric cancer using EFTUD2 Polyclonal Antibody (Product # PA5-96559) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EFTUD2 in paraffin-embedded mouse brain using EFTUD2 Polyclonal Antibody (Product # PA5-96559) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of EFTUD2 in paraffin-embedded mouse kidney using EFTUD2 Polyclonal Antibody (Product # PA5-96559) at a dilution of 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of EFTUD2 was performed in 100 µg extracts of 293T cells using EFTUD2 Polyclonal Antibody (Product # PA5-96559). Western blot was performed from the immunoprecipitate using EFTUD2 Polyclonal Antibody.