SP7411

antibody from Acris Antibodies GmbH

Targeting: BAG2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

SP7411

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#SP7411, RRID:AB_973147

Product name

anti BAG2

Antibody type

Polyclonal

Antigen

Full-length recombinant human BAG-2 protein

Reactivity

Human

Host

Rabbit

Vial size

50 µl

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using BAG2 antibody. Western blot analysis of BAG-2 using SP7411 at 1:2000. HeLa stably transfected Myc-tagged CHIP (carboxyl terminus of Hsp70-interacting protein) were immunoprecipitated with an anti-Myc antibody. Immunocomplexes were resolved by SDS-PAGE followed by western blot analysis of BAG-2. This experiment was done to show the colocalization of CHIP and Bag-2. Please refer to Dai et al (2005) for additional information on HeLa cells stably transfected with Myc-CHIP.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence using BAG2 antibody (SP7411). Immunofluorescence confocal microscopy of BAG-2 using SP7411 at 1/2000. HeLa cells stably transfected with Myc-CHIP (carboxyl terminus of Hsp70-interacting protein) and growing on coverslips were left untreated (A) or heat shocked at 42 degrees for 30 min (B). Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG-2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock. Please refer to Dai et al (2005) for additional information on HeLa cells stably transfected with Myc-CHIP.