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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Other assay [1]
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- Product number
- H00002160-M01 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- F11 Monoclonal Antibody (2H8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: SIPVFCHSSF YHDTDFLGEE LDIVAAKSHE ACQKLCTNAV RCQFFTYTPA QASCNEGKGK CYLKLSSNGS PTKILHGRGG ISGYTLRLCK MDNECTTKIK
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2H8
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of F11 expression in transfected 293T cell line by F11 monoclonal antibody (M01), clone 2H8.Lane 1: F11 transfected lysate(70.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged F11 is approximately 10 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of F11 transfected lysate using anti-F11 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F11 MaxPab rabbit polyclonal antibody.
