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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-96508 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KHDRBS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: TAPSRGRGGA IPPPPPPGRG VLTPRGSTVT RGALPVPPVA RGVPTPRARG APTVPGYRAP PPPAHEAYEE YGYDDGYGGE YDDQTYETYD NSYATQTQSV PEYYDYGHGV SEDAYDSYAP EEWATTRSSL KAPPQRSARG GYREHPYGRY; Positive Samples: HeLa, 293T, Jurkat, K-562; Cellular Location: Nucleus
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Development of a 3 RNA Binding Protein Signature for Predicting Prognosis and Treatment Response for Glioblastoma Multiforme.
Sun R, Pan Y, Mu L, Ma Y, Shen H, Long Y
Frontiers in genetics 2021;12:768930
Frontiers in genetics 2021;12:768930
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using KHDRBS2 Polyclonal antibody (Product # PA5-96508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of KHDRBS2 in extracts of various cell lines using KHDRBS2 Polyclonal Antibody (Product # PA5-96508) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KHDRBS2 in paraffin-embedded human prostate cancer using KHDRBS2 Polyclonal Antibody (Product # PA5-96508) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KHDRBS2 in paraffin-embedded human esophagus using KHDRBS2 Polyclonal Antibody (Product # PA5-96508) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of KHDRBS2 in paraffin-embedded human stomach using KHDRBS2 Polyclonal Antibody (Product # PA5-96508) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 9 Validation of the differential expression of the 3 RBPs in GBM cells. (A-D) Western blot images (A) and the relevant quantification of BICC1 (B) , GNL3L (C) , and KHDRBS2 (D) in GBM cell line U-87, primary GBM cells (GBM-1 and GBM-2), and normal glial cell line HEB. The relative expression of target proteins is quantified in comparison with beta-Actin and normalized to the corresponding expression in HEB cells. Data are shown as mean +- SEM from five independent experiments, * p < 0.05, ** p < 0.01, *** p < 0.001.
