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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- RQ6021 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Rex1 Antibody / ZFP42
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Storage
- After reconstitution, the ZFP42 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) placenta, 2) HeLa, 3) Caco-2 and 4) 22RV1 lysate with ZFP42 antibody. Expected molecular weight: 35-40 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human renal cancer with ZFP42 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human renal cancer with ZFP42 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human prostate cancer with ZFP42 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human a431 cells with ZFP42 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ZFP42 antibody.
