Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Other assay [1]
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Validation data
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- Product number
- 11546-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NMT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MADESETAV KPPAPPLPQM MEGNGNGHEH CSDCENEEDN SYNRGGLSPA NDTGAKKKKK KQKKKKEKGS ETDSAQDQPV KMNSLPAERI QEIQKAIELF SVGQGPAKTM EEASKRSYQF WDTQPVPKLG EVVNTHGPVE PDKDNIRQEP YTLPQGFTWD ALDLGDRGVL KELYTLLNEN YVEDDDNMFR FDYSPEFLLW ALRPPGWLPQ WHCGVRVVSS RKLVGFISAI PANIHIYDTE KKMVEINFLC VHKKLRSKRV APVLIREITR RVHLEGIFQA VYTAGVVLPK PVGTCRYWHR SL (1-301 aa encoded by BC006569)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human kidney tissue were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using NMT1 antibody 11546-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using NMT1 antibody 11546-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-NMT1 (IP:11546-1-AP, 4ug; Detection:11546-1-AP 1:500) with HeLa cells lysate 2000ug.