NBP1-82548

antibody from Novus Biologicals

Targeting: NMT1 NMT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-82548

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-82548, RRID:AB_11015528

Product name

Rabbit Polyclonal NMT1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human NMT1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NMT1 Antibody [NBP1-82548] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NMT1 Antibody [NBP1-82548] - Analysis in control (vector only transfected HEK293T lysate) and NMT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NMT1 Antibody [NBP1-82548] - Analysis using Anti-NMT1 antibody NBP1-82548 (A) shows similar pattern to independent antibody NBP1-82547 (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NMT1 Antibody [NBP1-82548] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons and neuropil.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NMT1 Antibody [NBP1-82548] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NMT1 Antibody [NBP1-82548] - Staining of human liver shows no positivity in hepatocytes as expected.