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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX127932 - Provider product page
- Provider
- GeneTex
- Product name
- NFAT1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Lovastatin blocks Kv1.3 channel in human T cells: a new mechanism to explain its immunomodulatory properties.
Zhao N, Dong Q, Qian C, Li S, Wu QF, Ding D, Li J, Wang BB, Guo KF, Xie JJ, Cheng X, Liao YH, Du YM
Scientific reports 2015 Nov 30;5:17381
Scientific reports 2015 Nov 30;5:17381
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- NFAT1 antibody detects NFATC2 protein by western blot analysis.A. 30 ?g BCL-1 whole cell lysate/extractB. 30 ?g Raw264.7 whole cell lysate/extract7.5% SDS-PAGENFAT1 antibody (GTX127932) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NFAT1 antibody detects NFATC2 protein by western blot analysis.A. 30 ?g Jurkat whole cell lysate/extractB. 30 ?g Raji whole cell lysate/extract7.5% SDS-PAGENFAT1 antibody (GTX127932) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membranes were blotted with NFAT1 antibody (GTX127932) diluted at 1:500 and competitor's antibody (MA1-025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NFAT1 antibody detects NFAT1 protein at cytoplasm by immunofluorescent analysis.Sample: RAW264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NFAT1 protein stained by NFAT1 antibody (GTX127932) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- NFAT1 antibody immunoprecipitates NFAT1 protein in IP experiments.IP samples: Raji whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of NFAT1 protein by 4 £gg NFAT1 antibody (GTX127932)7.5 % SDS-PAGEThe immunoprecipitated NFAT1 protein was detected by NFAT1 antibody (GTX127932) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
