sc-7296

antibody from Santa Cruz Biotechnology

Targeting: NFATC2 NF-ATP, NFAT1, NFATp

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-7296

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-7296, RRID:AB_628012

Product name

Anti-NFATC2

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

References

Submitted references

Complementary quantitative proteomics reveals that transcription factor AP-4 mediates E-box-dependent complex formation for transcriptional repression of HDM2.

Ku WC, Chiu SK, Chen YJ, Huang HH, Wu WG, Chen YJ
Molecular & cellular proteomics : MCP 2009 Sep;8(9):2034-50

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Secreted frizzle-related protein 2 stimulates angiogenesis via a calcineurin/NFAT signaling pathway.

Courtwright A, Siamakpour-Reihani S, Arbiser JL, Banet N, Hilliard E, Fried L, Livasy C, Ketelsen D, Nepal DB, Perou CM, Patterson C, Klauber-Demore N
Cancer research 2009 Jun 1;69(11):4621-8

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Integrin alpha6beta4 controls the expression of genes associated with cell motility, invasion, and metastasis, including S100A4/metastasin.

Chen M, Sinha M, Luxon BA, Bresnick AR, O'Connor KL
The Journal of biological chemistry 2009 Jan 16;284(3):1484-94

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Clofazimine inhibits human Kv1.3 potassium channel by perturbing calcium oscillation in T lymphocytes.

Ren YR, Pan F, Parvez S, Fleig A, Chong CR, Xu J, Dang Y, Zhang J, Jiang H, Penner R, Liu JO
PloS one 2008;3(12):e4009

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A scaffold protein, AHNAK1, is required for calcium signaling during T cell activation.

Matza D, Badou A, Kobayashi KS, Goldsmith-Pestana K, Masuda Y, Komuro A, McMahon-Pratt D, Marchesi VT, Flavell RA
Immunity 2008 Jan;28(1):64-74

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Cholecystokinin activates pancreatic calcineurin-NFAT signaling in vitro and in vivo.

Gurda GT, Guo L, Lee SH, Molkentin JD, Williams JA
Molecular biology of the cell 2008 Jan;19(1):198-206

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Elevated intracellular calcium increases ferritin H expression through an NFAT-independent post-transcriptional mechanism involving mRNA stabilization.

MacKenzie EL, Tsuji Y
The Biochemical journal 2008 Apr 1;411(1):107-13

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Targeting calcineurin activation as a therapeutic strategy for T-cell acute lymphoblastic leukemia

Hind Medyouf, Hélène Alcalde, Caroline Berthier, Marie Claude Guillemin, Nuno R dos Santos, Anne Janin, Didier Decaudin, Hugues de Thé, Jacques Ghysdael
Nature Medicine 2007 May;13(6):736-741

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Calcineurin-GATA-6 pathway is involved in smooth muscle-specific transcription.

Wada H, Hasegawa K, Morimoto T, Kakita T, Yanazume T, Abe M, Sasayama S
The Journal of cell biology 2002 Mar 18;156(6):983-91

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 100

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in lymphoid cells outside reaction centra.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.