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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX110497 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110497, RRID:AB_1951227
- Product name
- PIASy antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PIASy antibody detects PIASy protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of human PIAS4-transfected 293T cells10% SDS-PAGEPIASy antibody (GTX110497) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PIASy(GTX110497) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using PIAS4(GTX110497) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PIASy antibody detects PIASy protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. PIASy antibody (GTX110497) diluted at 1:500.
