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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX116181 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116181, RRID:AB_10626277
- Product name
- PIASy antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PIASy antibody detects PIASy protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of human PIAS4-transfected 293T cells12 % SDS-PAGEPIASy antibody (GTX116181) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PIASy antibody detects PIAS4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PIAS4 protein stained by PIASy antibody (GTX116181) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Hela xenograft, using PIASy(GTX116181) antibody at 1:500 dilution.
