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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2258 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2258, RRID:AB_1578740
- Product name
- PIAS4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PIAS4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Regulation of transforming growth factor-beta signaling by protein inhibitor of activated STAT, PIASy through Smad3.
A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy through its PIASy-enhanced modification by SUMO-1 or SUMO-3.
Modification of GATA-2 transcriptional activity in endothelial cells by the SUMO E3 ligase PIASy.
Imoto S, Sugiyama K, Muromoto R, Sato N, Yamamoto T, Matsuda T
The Journal of biological chemistry 2003 Sep 5;278(36):34253-8
The Journal of biological chemistry 2003 Sep 5;278(36):34253-8
A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy through its PIASy-enhanced modification by SUMO-1 or SUMO-3.
Subramanian L, Benson MD, Iñiguez-Lluhí JA
The Journal of biological chemistry 2003 Mar 14;278(11):9134-41
The Journal of biological chemistry 2003 Mar 14;278(11):9134-41
Modification of GATA-2 transcriptional activity in endothelial cells by the SUMO E3 ligase PIASy.
Chun TH, Itoh H, Subramanian L, Iñiguez-Lluhí JA, Nakao K
Circulation research 2003 Jun 13;92(11):1201-8
Circulation research 2003 Jun 13;92(11):1201-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The PIAS4 polyclonal antibody (Cat # PAB2258) is used in Western blot to detect recombinant GST-PIAS4 in bacterial cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the PIAS4 polyclonal antibody (Cat # PAB2258) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
