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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-29146 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIASy Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: human PIAS4-transfected 293T.
- Concentration
- 1 mg/mL
Submitted references Salmonella Engages Host MicroRNAs To Modulate SUMOylation: a New Arsenal for Intracellular Survival.
Verma S, Mohapatra G, Ahmad SM, Rana S, Jain S, Khalsa JK, Srikanth CV
Molecular and cellular biology 2015 Sep 1;35(17):2932-46
Molecular and cellular biology 2015 Sep 1;35(17):2932-46
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PIASy Polyclonal Antibody detects PIASy protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human PIAS4-transfected 293T cells.10% SDS-PAGE. PIASy Polyclonal Antibody (Product # PA5-29146) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PIASy in paraformaldehyde-fixed HeLa cells using a PIASy polyclonal antibody (Product # PA5-29146) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PIASy was performed in paraffin-embedded mouse colon tissue using PIASy Polyclonal Antibody (Product # PA5-29146) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using PIAS4 (Product # PA5-29146) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
