12664-1-AP

antibody from Invitrogen Antibodies

Targeting: SPPL2C IMP5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12664-1-AP

Provider

Invitrogen Antibodies

Product name

IMP5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: QELSLFWTG QGRAKMCGLG CAPSAGSRQK QEGAADAHTA STLERGTSRG AGDLDSNPGE DTTEIVTISE NEATNPEDRS DSSEGWSDAH LDPNELPFIP PGASEELVPL MPMAMLIPLM PLMPPPSELG HVHAQAQAHE TGLPWAGLHK RKGLKVRKSM STQAPL (520-684 aa encoded by BC025401)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.18 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12664-1-AP (IMP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12664-1-AP (IMP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HEK-293 cells using 12664-1-AP (IMP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 12664-1-AP ( IMP5 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 12664-1-AP ( IMP5 Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-IMP5 (IP:12664-1-AP, 4ug; Detection:12664-1-AP 1:500) with HEK-293 cells lysate 3600ug.