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Learn26759-1-AP
antibody from Invitrogen Antibodies
Targeting: SUFU
PRO1280, SUFUH, SUFUXL
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 26759-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SUFU Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MQPVQTPFG VVTFLQIVGV CTEELHSAQQ WNGQGILELL RTVPIAGGPW LITDMRRGET IFEIDPHLQE RVDKGIETDG SNLSGVSAKC AWDDLSRPPE DDEDSRSICI GTQPRRLSGK DTEQIRETLR RGLEINSKPV LPPINPQRQN GLAHDRAPSR KDSLESDSST AIIPHELIRT RQLESVHLKF NQESGALIPL CLRGRLLHGR HFTYKSITGD MAITFVSTGV EGAFATEEHP YAAHGPWLQI LLTEEFVEKM LEDLEDLTSP E (185-454 aa encoded by BC013291)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.43 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26759-1-AP (SUFU Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 26759-1-AP (SUFU Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C6 cells were subjected to SDS PAGE followed by western blot with 26759-1-AP ( SUFU Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 26759-1-AP (SUFU antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 26759-1-AP ( SUFU Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 26759-1-AP ( SUFU Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HEK-293 cells were intracellularly stained with 0.20 µg/test Anti-Human SUFU (Product # 26759-1-AP) (red) or 0.20 µg control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1,000. Fixed with90% MeOH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SUFU (IP:26759-1-AP, 4ug; Detection:26759-1-AP 1:1000) with NIH/3T3 cells lysate 3600ug.
