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LearnH00055662-M01
antibody from Abnova Corporation
Targeting: HIF1AN
DKFZp762F1811, FIH1, FLJ20615, FLJ22027
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00055662-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00055662-M01, RRID:AB_606382
- Product name
- HIF1AN monoclonal antibody (M01), clone 1D8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant HIF1AN.
- Antigen sequence
MAATAAEAVASGSGEPREEAGALGPAWDESQLRSY
SFPTRPIPRLSQSDPRAEELIENEEPVVLTDTNLV
YPALKWDLEYLQENIGNGDFSVYSASTHKFLYYDE
KKMANFQNFKPRSNREEMKFHEFVEKLQDIQQRGG
EERLYLQQTLNDTVGRKIVMDFLGFNWNWINKQQG
KRGWGQLTSNLLLIGMEGNVTPAHYDEQQNFFAQI
KGYKRCILFPPDQFECLYPYPVHHPCDRQSQVDFD
NPDYERFPNFQNVVGYETVVGPGDVLYIPMYWWHH
IESLLNGGITITVNFWYKGAPTPKRIEYPLKAHQK
VAIMRNIEKMLGEALGNPQEVGPLLNTMIKGRYN- Isotype
- IgG
- Antibody clone number
- 1D8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HIF1AN monoclonal antibody (M01), clone 1D8 Western Blot analysis of HIF1AN expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of HIF1AN expression in transfected 293T cell line by HIF1AN monoclonal antibody (M01), clone 1D8.Lane 1: HIF1AN transfected lysate (Predicted MW: 40.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged HIF1AN is 0.1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to HIF1AN on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of HIF1AN transfected lysate using anti-HIF1AN monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HIF1AN monoclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
