Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00007343-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007343-M01, RRID:AB_548994
- Product name
- Mouse Monoclonal UBTF Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. UBTF - upstream binding transcription factor, RNA polymerase I
- Reactivity
- Human, Mouse, Bovine, Porcine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Three-Dimensional Distribution of UBF and Nopp140 in Relationship to Ribosomal DNA Transcription During Mouse Preimplantation Development.
The maternal nucleolus plays a key role in centromere satellite maintenance during the oocyte to embryo transition.
Nucleolar localization of GLTSCR2/PICT-1 is mediated by multiple unique nucleolar localization sequences.
Identification and characterization of a novel ubiquitous nucleolar protein 'NARR' encoded by a gene overlapping the rab34 oncogene.
Role of ooplasm in nuclear and nucleolar remodeling of intergeneric somatic cell nuclear transfer embryos during the first cell cycle.
Nuclear and nucleolar reprogramming during the first cell cycle in bovine nuclear transfer embryos.
Nucleologenesis and embryonic genome activation are defective in interspecies cloned embryos between bovine ooplasm and rhesus monkey somatic cells.
Koné MC, Fleurot R, Chebrout M, Debey P, Beaujean N, Bonnet-Garnier A
Biology of reproduction 2016 Apr;94(4):95
Biology of reproduction 2016 Apr;94(4):95
The maternal nucleolus plays a key role in centromere satellite maintenance during the oocyte to embryo transition.
Fulka H, Langerova A
Development (Cambridge, England) 2014 Apr;141(8):1694-704
Development (Cambridge, England) 2014 Apr;141(8):1694-704
Nucleolar localization of GLTSCR2/PICT-1 is mediated by multiple unique nucleolar localization sequences.
Kalt I, Levy A, Borodianskiy-Shteinberg T, Sarid R
PloS one 2012;7(1):e30825
PloS one 2012;7(1):e30825
Identification and characterization of a novel ubiquitous nucleolar protein 'NARR' encoded by a gene overlapping the rab34 oncogene.
Zougman A, Mann M, Wisniewski JR
Nucleic acids research 2011 Sep 1;39(16):7103-13
Nucleic acids research 2011 Sep 1;39(16):7103-13
Role of ooplasm in nuclear and nucleolar remodeling of intergeneric somatic cell nuclear transfer embryos during the first cell cycle.
Østrup O, Strejcek F, Petrovicova I, Lucas-Hahn A, Morovic M, Lemme E, Petersen B, Laurincikova N, Niemann H, Laurincik J, Hyttel P
Cellular reprogramming 2011 Apr;13(2):145-55
Cellular reprogramming 2011 Apr;13(2):145-55
Nuclear and nucleolar reprogramming during the first cell cycle in bovine nuclear transfer embryos.
Østrup O, Petrovicova I, Strejcek F, Morovic M, Lucas-Hahn A, Lemme E, Petersen B, Niemann H, Laurincik J, Maddox-Hyttel P
Cloning and stem cells 2009 Sep;11(3):367-75
Cloning and stem cells 2009 Sep;11(3):367-75
Nucleologenesis and embryonic genome activation are defective in interspecies cloned embryos between bovine ooplasm and rhesus monkey somatic cells.
Song BS, Lee SH, Kim SU, Kim JS, Park JS, Kim CH, Chang KT, Han YM, Lee KK, Lee DS, Koo DB
BMC developmental biology 2009 Jul 28;9:44
BMC developmental biology 2009 Jul 28;9:44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBTF Antibody (6B6) [H00007343-M01] - UBTF monoclonal antibody (M01), clone 6B6 Analysis of UBTF expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: UBTF Antibody (6B6) [H00007343-M01] - Detection limit for recombinant GST tagged UBTF is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: UBTF Antibody (6B6) [H00007343-M01] - Analysis of monoclonal antibody to UBTF on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UBTF Antibody (6B6) [H00007343-M01] - Analysis of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.