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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-82164 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82164, RRID:AB_11005407
- Product name
- Rabbit Polyclonal C14orf119 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human C14orf119 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP).
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Fagerberg L, Oksvold P, Skogs M, Algenäs C, Lundberg E, Pontén F, Sivertsson A, Odeberg J, Klevebring D, Kampf C, Asplund A, Sjöstedt E, Al-Khalili Szigyarto C, Edqvist PH, Olsson I, Rydberg U, Hudson P, Ottosson Takanen J, Berling H, Björling L, Tegel H, Rockberg J, Nilsson P, Navani S, Jirström K, Mulder J, Schwenk JM, Zwahlen M, Hober S, Forsberg M, von Feilitzen K, Uhlén M
Journal of proteome research 2013 Jun 7;12(6):2439-48
Journal of proteome research 2013 Jun 7;12(6):2439-48
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy.
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of proteomics 2012 Apr 3;75(7):2236-51
Journal of proteomics 2012 Apr 3;75(7):2236-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: C14orf119 Antibody [NBP1-82164] - Analysis in control (vector only transfected HEK293T lysate) and C14orf119 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: C14orf119 Antibody [NBP1-82164] - Staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
