Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF3669 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Wnt-9b Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Wnt-9b in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Wnt-2, rhWnt-9a, recombinant mouse (rm) Wnt-1, rmWnt-2b, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-11, and rmWnt-16 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions.
Wnt signaling in the pathogenesis of human HIV-associated pain syndromes.
Nowialis P, Lopusna K, Opavska J, Haney SL, Abraham A, Sheng P, Riva A, Natarajan A, Guryanova O, Simpson M, Hlady R, Xie M, Opavsky R
Nature communications 2019 Sep 26;10(1):4374
Nature communications 2019 Sep 26;10(1):4374
Wnt signaling in the pathogenesis of human HIV-associated pain syndromes.
Shi Y, Shu J, Gelman BB, Lisinicchia JG, Tang SJ
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2013 Sep;8(4):956-64
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2013 Sep;8(4):956-64
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Wnt-9b in Mouse Embryo. Wnt-9b was detected in imm-ersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Wnt-9b Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF3669) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to the retina. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.