AF3669

antibody from R&D Systems

Targeting: WNT9B WNT14B, WNT15

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3669

Provider

R&D Systems

Product name

Mouse Wnt-9b Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse Wnt-9b in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Wnt-2, rhWnt-9a, recombinant mouse (rm) Wnt-1, rmWnt-2b, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-11, and rmWnt-16 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

O35468


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Wnt signaling in the pathogenesis of human HIV-associated pain syndromes.

Shi Y, Shu J, Gelman BB, Lisinicchia JG, Tang SJ
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2013 Sep;8(4):956-64

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Wnt-9b in Mouse Embryo. Wnt-9b was detected in imm-ersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Wnt-9b Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF3669) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to the retina. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.