Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF3669 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Wnt-9b Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Wnt-9b in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Wnt-2, rhWnt-9a, recombinant mouse (rm) Wnt-1, rmWnt-2b, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-11, and rmWnt-16 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O35468
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Wnt signaling in the pathogenesis of human HIV-associated pain syndromes.
Shi Y, Shu J, Gelman BB, Lisinicchia JG, Tang SJ
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2013 Sep;8(4):956-64
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2013 Sep;8(4):956-64
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Wnt-9b in Mouse Embryo. Wnt-9b was detected in imm-ersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Wnt-9b Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF3669) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to the retina. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.