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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB3669 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Wnt-9b Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Wnt-9b in Western blots. Detects recombinant mouse Wnt-9b in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Wnt-2, -6, -7a, -7b, -9a, recombinant mouse Wnt-1, -2b, -4, -5a, -5b, -8a, -8b, -10a, -10b, -11, or -16 is observed.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
O35468.2- Isotype
- IgG
- Antibody clone number
- 406612
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human/Mouse Wnt-9b by Western Blot. Western blot shows lysates of KATO-III human gastric carcinoma cell line, T47D human breast cancer cell line, and ME-180 human cervical epithelial carcinoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Human/Mouse Wnt-9b Monoclonal Antibody (Catalog # MAB3669) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human Wnt-9b (20 ng) was included. A specific band for native Wnt-9b was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
