Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AGC-028-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- mGluR8 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6): - 1-3. Anti-mGluR8 (extracellular) Antibody (#AGC-028), (1:400).4-6. Anti-mGluR8 (extracellular) Antibody , preincubated with mGluR8 (extracellular) Blocking Peptide (#BLP-GC028).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6): - 1-3. Anti-mGluR8 (extracellular) Antibody (#AGC-028), (1:400).4-6. Anti-mGluR8 (extracellular) Antibody , preincubated with mGluR8 (extracellular) Blocking Peptide (#BLP-GC028).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of mGluR8 in rat globus pallidus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-mGluR8 (extracellular) Antibody (#AGC-028), (1:100). A. Staining reveals expression of mGluR8 (red) in globus pallidus neurons (arrow) and their dendrites. B. The same section was also stained with DAPI (blue) revealing cell nuclei. C. Merge of the two images.