Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-76657 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-76657, RRID:AB_11016404
- Product name
- Rabbit Polyclonal Endo G Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Bupivacaine induces apoptosis through caspase-dependent and -independent pathways in canine mammary tumor cells.
Chiu YS, Cheng YH, Lin SW, Chang TS, Liou CJ, Lai YS
Research in veterinary science 2015 Jun;100:232-8
Research in veterinary science 2015 Jun;100:232-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Endo G Antibody [NBP1-76657] - Analysis of Endo G in mouse (M) 3T3 and human (H) HepG2 cell lysates with anti-Endo G using NBP1-76657 at 2 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human Endo G recombinant protein per lane. Antibodies: Endo G NBP1-76657, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL Lane 2: 1 ug/mL Lane 3: 2 ug/mL
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation in Human A431 Cell Lysate with the presence (A) or absence (B and C) of blocking peptide. Loading: 15 ug of lysates per lane. Antibodies: Endo G NBP1-76657 (A: 0.5 ug/mL, B: 0.5 ug/mL, C: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Endo G Antibody [NBP1-76657] - Western Blot Validation in Mouse 3T3 (M) and Human HepG2 (H) cell lysates. Loading: 15 ug of lysates per lane. Antibodies: Endo G NBP1-76657 (2 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Endo G Antibody [NBP1-76657] - Human pancreas with anti-Endo G at 15 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Endo G Antibody [NBP1-76657] - Analysis of human pancreas tissue with EndoG antibody at 2.5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Endo G Antibody [NBP1-76657] - Analysis of human pancreas tissue with EndoG antibody at 2.5 ug/ml.