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LearnF40202
antibody from NSJ Bioreagents
Targeting: PRDM2
HUMHOXY1, KMT8, KMT8A, MTB-ZF, RIZ, RIZ1, RIZ2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [3]
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Validation data
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- Product number
- F40202 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- RIZ1 Antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant GST fusion protein encoding aa 1-347 of human RIZ1. This RIZ1 antibody is specific and does not recognize the beta isoform (RIZ2).
- Description
- Purified antibody
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 33AT1045
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the RIZ1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of PRDM2 / RIZ1 antibody and transfected cell lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ChIP analysis: soluble chromatin was prepared from MCF7 cells treated/untreated with E2 for 45 min. Immunoprecipitation was performed with RIZ1 antibody. DNA extractions amplified using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ChIP: Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed with RIZ1 antibody and control immunoglobulin G as indicated.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts with RIZ1 antibody. Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the Cdc25c promoter region.
