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LearnMAB1151
antibody from Abnova Corporation
Targeting: PRDM2
HUMHOXY1, KMT8, KMT8A, MTB-ZF, RIZ, RIZ1, RIZ2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- MAB1151 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1151, RRID:AB_1579080
- Product name
- PRDM2 monoclonal antibody, clone 33AT1045
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant PRDM2.
- Isotype
- IgG
- Antibody clone number
- 33AT1045
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Genotypes and haplotypes of the estrogen receptor genes, but not the retinoblastoma-interacting zinc finger protein 1 gene, are associated with osteoporosis.
DNA methylation of the RIZ1 tumor suppressor gene plays an important role in the tumorigenesis of cervical cancer.
Genetic variants of methyl metabolizing enzymes and epigenetic regulators: associations with promoter CpG island hypermethylation in colorectal cancer.
Harsløf T, Husted LB, Carstens M, Stenkjaer L, Langdahl BL
Calcified tissue international 2010 Jul;87(1):25-35
Calcified tissue international 2010 Jul;87(1):25-35
DNA methylation of the RIZ1 tumor suppressor gene plays an important role in the tumorigenesis of cervical cancer.
Cheng HY, Gao Y, Lou G
European journal of medical research 2010 Jan 29;15(1):20-4
European journal of medical research 2010 Jan 29;15(1):20-4
Genetic variants of methyl metabolizing enzymes and epigenetic regulators: associations with promoter CpG island hypermethylation in colorectal cancer.
de Vogel S, Wouters KA, Gottschalk RW, van Schooten FJ, de Goeij AF, de Bruïne AP, Goldbohm RA, van den Brandt PA, Weijenberg MP, van Engeland M
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2009 Nov;18(11):3086-96
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2009 Nov;18(11):3086-96
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PRDM2 monoclonal antibody, clone 33AT1045 (Cat # MAB1151) in lysate from transformed cells. Lane A : mock plasmid, Lane B : PRDM2 [1-347]-encoding plasmid. PRDM2 [1-347] (arrow) was detected using purified monoclonal antibody. Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- A : Soluble chromatin was prepared from MCF-7 cells not treated or treated with E2 for 45 min. Immunoprecipitation was performed with PRDM2 monoclonal antibody, clone 33AT1045 (Cat # MAB1151). DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter.B : Time course analysis of PRDM2 binding to the pS2 gene promoter. MCF-7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis.
- Validation comment
- Immunoprecipitation
