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LearnMA5-11144
antibody from Invitrogen Antibodies
Targeting: PRDM2
HUMHOXY1, KMT8, KMT8A, MTB-ZF, RIZ, RIZ1, RIZ2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [3]
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- Product number
- MA5-11144 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRDM2 Monoclonal Antibody (33AT1045)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody is specific for RIZ1, it does not recognize the isoform beta (RIZ2).
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 33AT1045
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a PRDM2 monoclonal antibody (Product # MA5-11144) in lysate from PRDM2 transgenic lysate. Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP analysis of estrogen target genes. (A) Soluble chromatin was prepared from MCF-7 cells either treated or untreated with E2 for 45 min. Immunoprecipitation was performed with a RIZ1 mononclonal antibody (Product # MA5-11144). DNA extractions were amplified by using primer sets that cover the pS2 gene promoter region or the GAPDH gene promoter.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP analysis of estrogen target genes. (B) Time course analysis of RIZ1 binding to the pS2 gene promoter. MCF-7 cells treated with E2 for different periods of time, as indicated at the top of each lane, were processed for ChIP analysis. Immunoprecipitation was performed with RIZ1 monoclonal antibody (Product # MA5-11144) and control IgG as indicated.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP analysis of estrogen target genes. (C) ChIP analysis was performed on RIZ1 knockout mouse embryonic fibroblasts by using a RIZ1 monoclonal antibody (Product # MA5-11144). Immunoprecipitated chromatin DNA was analyzed by PCR with primers in the G6pd promoter region and in the cdc25c promoter region.
