GTX121929

antibody from GeneTex

Targeting: NOX4 KOX, KOX-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX121929

Provider

GeneTex

Proper citation

GeneTex Cat#GTX121929, RRID:AB_10726092

Product name

NOX4 antibody [N3C3]

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Kidney dysfunction in the low-birth weight murine adult: implications of oxidative stress.

Abdulmahdi W, Rabadi MM, Jules E, Marghani Y, Marji N, Leung J, Zhang F, Siani A, Siskind T, Vedovino K, Chowdhury N, Sekulic M, Ratliff BB
American journal of physiology. Renal physiology 2018 Sep 1;315(3):F583-F594

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Gallic Acid Reduces Blood Pressure and Attenuates Oxidative Stress and Cardiac Hypertrophy in Spontaneously Hypertensive Rats.

Jin L, Piao ZH, Sun S, Liu B, Kim GR, Seok YM, Lin MQ, Ryu Y, Choi SY, Kee HJ, Jeong MH
Scientific reports 2017 Nov 15;7(1):15607

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Rehydration with soft drink-like beverages exacerbates dehydration and worsens dehydration-associated renal injury.

García-Arroyo FE, Cristóbal M, Arellano-Buendía AS, Osorio H, Tapia E, Soto V, Madero M, Lanaspa MA, Roncal-Jiménez C, Bankir L, Johnson RJ, Sánchez-Lozada LG
American journal of physiology. Regulatory, integrative and comparative physiology 2016 Jul 1;311(1):R57-65

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MicroRNA-146a decreases high glucose/thrombin-induced endothelial inflammation by inhibiting NAPDH oxidase 4 expression.

Wang HJ, Huang YL, Shih YY, Wu HY, Peng CT, Lo WY
Mediators of inflammation 2014;2014:379537

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Neuronal NAD(P)H oxidases contribute to ROS production and mediate RGC death after ischemia.

Dvoriantchikova G, Grant J, Santos AR, Hernandez E, Ivanov D
Investigative ophthalmology & visual science 2012 May 14;53(6):2823-30

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: Jurkat 7.5% SDS PAGE GTX121929 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NOX4 antibody detects NOX4 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX4 antibody (GTX121929) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) unboiled 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX4 antibody [N3C3] (GTX121929) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NOX4 antibody [N3C3] detects NOX4 protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: NOX4 protein stained by NOX4 antibody [N3C3] (GTX121929) diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NOX4(GTX121929) antibody at 1:250 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NOX4 antibody [N3C3] detects NOX4 protein at cytoplasm in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. NOX4 antibody [N3C3] (GTX121929) diluted at 1:500.