NB110-58849

Kaushik D, Ashcraft KA, Wang H, Shanmugasundaram K, Shah PK, Gonzalez G, Nazarullah A, Tye CB, Liss MA, Pruthi DK, Mansour AM, Chowdhury W, Bacich D, Zhang H, Watson AL, Block K, O'Keefe D, Rodriguez R
Translational research : the journal of laboratory and clinical medicine 2020 Sep;223:1-14

Identification of highly potent and selective inhibitor, TIPTP, of the p22phox-Rubicon axis as a therapeutic agent for rheumatoid arthritis.

Kim YR, Kim JS, Gu SJ, Jo S, Kim S, Young Kim S, Lee D, Jang K, Choo H, Kim TH, Jung JU, Min SJ, Yang CS
Scientific reports 2020 Mar 12;10(1):4570

SIRT3, PP2A and TTP protein stability in the presence of TNF-α on vincristine-induced apoptosis of leukaemia cells.

Wang LJ, Chiou JT, Lee YC, Huang CH, Shi YJ, Chang LS
Journal of cellular and molecular medicine 2020 Feb;24(4):2552-2565

Dapagliflozin Prevents NOX- and SGLT2-Dependent Oxidative Stress in Lens Cells Exposed to Fructose-Induced Diabetes Mellitus.

Chen YY, Wu TT, Ho CY, Yeh TC, Sun GC, Kung YH, Wong TY, Tseng CJ, Cheng PW
International journal of molecular sciences 2019 Sep 5;20(18)

Acute Changes in NADPH Oxidase 4 in Early Post-Traumatic Osteoarthritis.

Wegner AM, Campos NR, Robbins MA, Haddad AF, Cunningham HC, Yik JHN, Christiansen BA, Haudenschild DR
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2019 Nov;37(11):2429-2436

Fructus Ligustri Lucidi preserves bone quality through the regulation of gut microbiota diversity, oxidative stress, TMAO and Sirt6 levels in aging mice.

Li L, Chen B, Zhu R, Li R, Tian Y, Liu C, Jia Q, Wang L, Tang J, Zhao D, Mo F, Liu Y, Li Y, Orekhov AN, Brömme D, Zhang D, Gao S
Aging 2019 Nov 12;11(21):9348-9368

The NADPH oxidase 4 is a major source of hydrogen peroxide in human granulosa-lutein and granulosa tumor cells.

Buck T, Hack CT, Berg D, Berg U, Kunz L, Mayerhofer A
Scientific reports 2019 Mar 5;9(1):3585

Blockade of L-type Ca²⁺ channel attenuates doxorubicin-induced cardiomyopathy via suppression of CaMKII-NF-κB pathway.

Ikeda S, Matsushima S, Okabe K, Ikeda M, Ishikita A, Tadokoro T, Enzan N, Yamamoto T, Sada M, Deguchi H, Morimoto S, Ide T, Tsutsui H
Scientific reports 2019 Jul 8;9(1):9850

Anagliptin protects neuronal cells against endogenous amyloid β (Aβ)-induced cytotoxicity and apoptosis.

Chen Z, Tao S, Li X, Zeng X, Zhang M, Yao Q
Artificial cells, nanomedicine, and biotechnology 2019 Dec;47(1):2213-2220

Mechanisms of Fasting-Mediated Protection against Renal Injury and Fibrosis Development after Ischemic Acute Kidney Injury.

Rojas-Morales P, Tapia E, León-Contreras JC, González-Reyes S, Jiménez-Osorio AS, Trujillo J, Pavón N, Granados-Pineda J, Hernández-Pando R, Sánchez-Lozada LG, Osorio-Alonso H, Pedraza-Chaverri J
Biomolecules 2019 Aug 22;9(9)

Hydrogen Sulfide Donor NaHS Improves Metabolism and Reduces Muscle Atrophy in Type 2 Diabetes: Implication for Understanding Sarcopenic Pathophysiology.

Bitar MS, Nader J, Al-Ali W, Al Madhoun A, Arefanian H, Al-Mulla F
Oxidative medicine and cellular longevity 2018;2018:6825452

NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas.

Tang P, Dang H, Huang J, Xu T, Yuan P, Hu J, Sheng JF
Scientific reports 2018 Oct 26;8(1):15897

Sulforaphane protects rabbit corneas against oxidative stress injury in keratoconus through activation of the Nrf-2/HO-1 antioxidant pathway.

Liu R, Yan X
International journal of molecular medicine 2018 Nov;42(5):2315-2328

NAD(P)H Oxidase Activity in the Small Intestine Is Predominantly Found in Enterocytes, Not Professional Phagocytes.

Lindquist RL, Bayat-Sarmadi J, Leben R, Niesner R, Hauser AE
International journal of molecular sciences 2018 May 4;19(5)

Isoquercetin attenuates oxidative stress and neuronal apoptosis after ischemia/reperfusion injury via Nrf2-mediated inhibition of the NOX4/ROS/NF-κB pathway.

Dai Y, Zhang H, Zhang J, Yan M
Chemico-biological interactions 2018 Mar 25;284:32-40

3,3'-Diindolylmethane enhances apoptosis in docetaxel-treated breast cancer cells by generation of reactive oxygen species.

Lanza-Jacoby S, Cheng G
Pharmaceutical biology 2018 Dec;56(1):407-414

A Novel Synthetic Material, BMM, Accelerates Wound Repair by Stimulating Re-Epithelialization and Fibroblast Activation.

Seo GY, Hyun C, Koh D, Park S, Lim Y, Kim YM, Cho M
International journal of molecular sciences 2018 Apr 11;19(4)

Antioxidant Effect of Fructus Ligustri Lucidi Aqueous Extract in Ovariectomized Rats Is Mediated through Nox4-ROS-NF-κB Pathway.

Wang L, Ma R, Guo Y, Sun J, Liu H, Zhu R, Liu C, Li J, Li L, Chen B, Sun L, Tang J, Zhao D, Mo F, Niu J, Jiang G, Fu M, Brömme D, Zhang D, Gao S
Frontiers in pharmacology 2017;8:266

NOX1 and NOX4 are required for the differentiation of mouse F9 cells into extraembryonic endoderm.

Dickson BJ, Gatie MI, Spice DM, Kelly GM
PloS one 2017;12(2):e0170812

NOX4 functions as a mitochondrial energetic sensor coupling cancer metabolic reprogramming to drug resistance.

Shanmugasundaram K, Nayak BK, Friedrichs WE, Kaushik D, Rodriguez R, Block K
Nature communications 2017 Oct 19;8(1):997

The mitochondrial-targeted peptide, SS-31, improves glomerular architecture in mice of advanced age.

Sweetwyne MT, Pippin JW, Eng DG, Hudkins KL, Chiao YA, Campbell MD, Marcinek DJ, Alpers CE, Szeto HH, Rabinovitch PS, Shankland SJ
Kidney international 2017 May;91(5):1126-1145

ROS-induced ROS release orchestrated by Nox4, Nox2, and mitochondria in VEGF signaling and angiogenesis.

Kim YM, Kim SJ, Tatsunami R, Yamamura H, Fukai T, Ushio-Fukai M
American journal of physiology. Cell physiology 2017 Jun 1;312(6):C749-C764

Subcellular localization of the FLT3-ITD oncogene plays a significant role in the production of NOX- and p22^phox-derived reactive oxygen species in acute myeloid leukemia.

Moloney JN, Stanicka J, Cotter TG
Leukemia research 2017 Jan;52:34-42

Targeting of NADPH oxidase in vitro and in vivo suppresses fibroblast activation and experimental skin fibrosis.

Dosoki H, Stegemann A, Taha M, Schnittler H, Luger TA, Schröder K, Distler JH, Kerkhoff C, Böhm M
Experimental dermatology 2017 Jan;26(1):73-81

Critical role of X-box binding protein 1 in NADPH oxidase 4-triggered cardiac hypertrophy is mediated by receptor interacting protein kinase 1.

Chen L, Zhao M, Li J, Wang Y, Bao Q, Wu S, Deng X, Tang X, Wu W, Liu X
Cell cycle (Georgetown, Tex.) 2017 Feb 16;16(4):348-359

Nuclear membrane-localised NOX4D generates pro-survival ROS in FLT3-ITD-expressing AML.

Moloney JN, Jayavelu AK, Stanicka J, Roche SL, O'Brien RL, Scholl S, Böhmer FD, Cotter TG
Oncotarget 2017 Dec 1;8(62):105440-105457

Reciprocal regulation of eNOS, H₂S and CO-synthesizing enzymes in human atheroma: Correlation with plaque stability and effects of simvastatin.

Sigala F, Efentakis P, Karageorgiadi D, Filis K, Zampas P, Iliodromitis EK, Zografos G, Papapetropoulos A, Andreadou I
Redox biology 2017 Aug;12:70-81

Azithromycin attenuates myofibroblast differentiation and lung fibrosis development through proteasomal degradation of NOX4.

Tsubouchi K, Araya J, Minagawa S, Hara H, Ichikawa A, Saito N, Kadota T, Sato N, Yoshida M, Kurita Y, Kobayashi K, Ito S, Fujita Y, Utsumi H, Yanagisawa H, Hashimoto M, Wakui H, Yoshii Y, Ishikawa T, Numata T, Kaneko Y, Asano H, Yamashita M, Odaka M, Morikawa T, Nakayama K, Nakanishi Y, Kuwano K
Autophagy 2017 Aug 3;13(8):1420-1434

The Expression of NOX4 in Smooth Muscles of Small Airway Correlates with the Disease Severity of COPD.

Liu X, Hao B, Ma A, He J, Liu X, Chen J
BioMed research international 2016;2016:2891810

Skeletal Muscle Regeneration and Oxidative Stress Are Altered in Chronic Kidney Disease.

Avin KG, Chen NX, Organ JM, Zarse C, O'Neill K, Conway RG, Konrad RJ, Bacallao RL, Allen MR, Moe SM
PloS one 2016;11(8):e0159411

Nox4 and Duox1/2 Mediate Redox Activation of Mesenchymal Cell Migration by PDGF.

Tyurin-Kuzmin PA, Zhdanovskaya ND, Sukhova AA, Sagaradze GD, Albert EA, Ageeva LV, Sharonov GV, Vorotnikov AV, Tkachuk VA
PloS one 2016;11(4):e0154157

Renoprotective Effects of a Highly Selective A3 Adenosine Receptor Antagonist in a Mouse Model of Adriamycin-induced Nephropathy.

Min HS, Cha JJ, Kim K, Kim JE, Ghee JY, Kim H, Lee JE, Han JY, Jeong LS, Cha DR, Kang YS
Journal of Korean medical science 2016 Sep;31(9):1403-12

NADPH oxidase-4 and MATER expressions in granulosa cells: Relationships with ovarian aging.

Maraldi T, Resca E, Nicoli A, Beretti F, Zavatti M, Capodanno F, Morini D, Palomba S, La Sala GB, De Pol A
Life sciences 2016 Oct 1;162:108-14

Osteopontin-stimulated apoptosis in cardiac myocytes involves oxidative stress and mitochondrial death pathway: role of a pro-apoptotic protein BIK.

Dalal S, Zha Q, Singh M, Singh K
Molecular and cellular biochemistry 2016 Jul;418(1-2):1-11

Gene silencing of endothelial von Willebrand Factor attenuates angiotensin II-induced endothelin-1 expression in porcine aortic endothelial cells.

Dushpanova A, Agostini S, Ciofini E, Cabiati M, Casieri V, Matteucci M, Del Ry S, Clerico A, Berti S, Lionetti V
Scientific reports 2016 Jul 22;6:30048

Elevation of cortical C26:0 due to the decline of peroxisomal β-oxidation potentiates amyloid β generation and spatial memory deficits via oxidative stress in diabetic rats.

Shi Y, Sun X, Sun Y, Hou L, Yao M, Lian K, Li J, Lu X, Jiang L
Neuroscience 2016 Feb 19;315:125-35

Metformin attenuates lung fibrosis development via NOX4 suppression.

Sato N, Takasaka N, Yoshida M, Tsubouchi K, Minagawa S, Araya J, Saito N, Fujita Y, Kurita Y, Kobayashi K, Ito S, Hara H, Kadota T, Yanagisawa H, Hashimoto M, Utsumi H, Wakui H, Kojima J, Numata T, Kaneko Y, Odaka M, Morikawa T, Nakayama K, Kohrogi H, Kuwano K
Respiratory research 2016 Aug 30;17(1):107

Regional Heterogeneity of Cerebral Microvessels and Brain Susceptibility to Oxidative Stress.

Austin SA, Santhanam AV, d'Uscio LV, Katusic ZS
PloS one 2015;10(12):e0144062

Metformin decreases growth of pancreatic cancer cells by decreasing reactive oxygen species: Role of NOX4.

Cheng G, Lanza-Jacoby S
Biochemical and biophysical research communications 2015 Sep 11;465(1):41-6

Knockdown of angiopoietin like-2 protects against angiotensin II-induced cerebral endothelial dysfunction in mice.

Yu C, Luo X, Duquette N, Thorin-Trescases N, Thorin E
American journal of physiology. Heart and circulatory physiology 2015 Mar 1;308(5):H386-97

Simvastatin Increases the Ability of Roflumilast N-oxide to Inhibit Cigarette Smoke-Induced Epithelial to Mesenchymal Transition in Well-differentiated Human Bronchial Epithelial Cells in vitro.

Milara J, Peiró T, Serrano A, Artigues E, Aparicio J, Tenor H, Sanz C, Cortijo J
COPD 2015 Jun;12(3):320-31

Epigenetic mechanisms regulate NADPH oxidase-4 expression in cellular senescence.

Sanders YY, Liu H, Liu G, Thannickal VJ
Free radical biology & medicine 2015 Feb;79:197-205

Bone Morphogenic Protein 4 Mediates NOX1-Dependent eNOS Uncoupling, Endothelial Dysfunction, and COX2 Induction in Type 2 Diabetes Mellitus.

Youn JY, Zhou J, Cai H
Molecular endocrinology (Baltimore, Md.) 2015 Aug;29(8):1123-33

Long-term treatment with the sodium glucose cotransporter 2 inhibitor, dapagliflozin, ameliorates glucose homeostasis and diabetic nephropathy in db/db mice.

Terami N, Ogawa D, Tachibana H, Hatanaka T, Wada J, Nakatsuka A, Eguchi J, Horiguchi CS, Nishii N, Yamada H, Takei K, Makino H
PloS one 2014;9(6):e100777

Inflammatory monocytes determine endothelial nitric-oxide synthase uncoupling and nitro-oxidative stress induced by angiotensin II.

Kossmann S, Hu H, Steven S, Schönfelder T, Fraccarollo D, Mikhed Y, Brähler M, Knorr M, Brandt M, Karbach SH, Becker C, Oelze M, Bauersachs J, Widder J, Münzel T, Daiber A, Wenzel P
The Journal of biological chemistry 2014 Oct 3;289(40):27540-50

Regulation of lipid accumulation by AMP-activated kinase [corrected] in high fat diet-induced kidney injury.

Declèves AE, Zolkipli Z, Satriano J, Wang L, Nakayama T, Rogac M, Le TP, Nortier JL, Farquhar MG, Naviaux RK, Sharma K
Kidney international 2014 Mar;85(3):611-23

Effects of Toll-like receptor antagonist 4,5-dihydro-3-phenyl-5-isoxasole acetic acid on the progression of kidney disease in mice on a high-fat diet.

Min HS, Kim JE, Lee MH, Song HK, Lee MJ, Lee JE, Kim HW, Cha JJ, Hyun YY, Han JY, Cha DR, Kang YS
Kidney research and clinical practice 2014 Mar;33(1):33-44

The fate of the primary cilium during myofibroblast transition.

Rozycki M, Lodyga M, Lam J, Miranda MZ, Fátyol K, Speight P, Kapus A
Molecular biology of the cell 2014 Mar;25(5):643-57

Decitabine induces delayed reactive oxygen species (ROS) accumulation in leukemia cells and induces the expression of ROS generating enzymes.

Fandy TE, Jiemjit A, Thakar M, Rhoden P, Suarez L, Gore SD
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Mar 1;20(5):1249-58

Dipeptidyl peptidase IV inhibitor protects against renal interstitial fibrosis in a mouse model of ureteral obstruction.

Min HS, Kim JE, Lee MH, Song HK, Kang YS, Lee MJ, Lee JE, Kim HW, Cha JJ, Chung YY, Hyun YY, Han JY, Cha DR
Laboratory investigation; a journal of technical methods and pathology 2014 Jun;94(6):598-607

Nox-derived ROS are acutely activated in pressure overload pulmonary hypertension: indications for a seminal role for mitochondrial Nox4.

Frazziano G, Al Ghouleh I, Baust J, Shiva S, Champion HC, Pagano PJ
American journal of physiology. Heart and circulatory physiology 2014 Jan 15;306(2):H197-205

Roflumilast N-oxide inhibits bronchial epithelial to mesenchymal transition induced by cigarette smoke in smokers with COPD.

Milara J, Peiró T, Serrano A, Guijarro R, Zaragozá C, Tenor H, Cortijo J
Pulmonary pharmacology & therapeutics 2014 Aug;28(2):138-48

Xanthine oxidase mediates hypoxia-inducible factor-2α degradation by intermittent hypoxia.

Nanduri J, Vaddi DR, Khan SA, Wang N, Makerenko V, Prabhakar NR
PloS one 2013;8(10):e75838

Nox4-derived ROS signaling contributes to TGF-β-induced epithelial-mesenchymal transition in pancreatic cancer cells.

Hiraga R, Kato M, Miyagawa S, Kamata T
Anticancer research 2013 Oct;33(10):4431-8

TGF-β1 stimulates mitochondrial oxidative phosphorylation and generation of reactive oxygen species in cultured mouse podocytes, mediated in part by the mTOR pathway.

Abe Y, Sakairi T, Beeson C, Kopp JB
American journal of physiology. Renal physiology 2013 Nov 15;305(10):F1477-90

Epithelial to mesenchymal transition is increased in patients with COPD and induced by cigarette smoke.

Milara J, Peiró T, Serrano A, Cortijo J
Thorax 2013 May;68(5):410-20

Inhibiting cancer metastasis via targeting NAPDH oxidase 4.

Zhang B, Liu Z, Hu X
Biochemical pharmacology 2013 Jul 15;86(2):253-66

Mitochondrial-localized NADPH oxidase 4 is a source of superoxide in angiotensin II-stimulated neurons.

Case AJ, Li S, Basu U, Tian J, Zimmerman MC
American journal of physiology. Heart and circulatory physiology 2013 Jul 1;305(1):H19-28

Deletion of angiotensin II type 1 receptor gene attenuates chronic alcohol-induced retinal ganglion cell death with preservation of VEGF expression.

Miao X, Lv H, Wang B, Chen Q, Miao L, Su G, Tan Y
Current eye research 2013 Jan;38(1):185-93

Combined losartan and nitro-oleic acid remarkably improves diabetic nephropathy in mice.

Liu Y, Jia Z, Liu S, Downton M, Liu G, Du Y, Yang T
American journal of physiology. Renal physiology 2013 Dec 1;305(11):F1555-62

Pleural mesothelial cell differentiation and invasion in fibrogenic lung injury.

Zolak JS, Jagirdar R, Surolia R, Karki S, Oliva O, Hock T, Guroji P, Ding Q, Liu RM, Bolisetty S, Agarwal A, Thannickal VJ, Antony VB
The American journal of pathology 2013 Apr;182(4):1239-47

Role of Nox2 in diabetic kidney disease.

You YH, Okada S, Ly S, Jandeleit-Dahm K, Barit D, Namikoshi T, Sharma K
American journal of physiology. Renal physiology 2013 Apr 1;304(7):F840-8

Deletion of angiotensin II type 1 receptor gene or scavenge of superoxide prevents chronic alcohol-induced aortic damage and remodelling.

Bai Y, Tan Y, Wang B, Miao X, Chen Q, Zheng Y, Cai L
Journal of cellular and molecular medicine 2012 Oct;16(10):2530-8

ROS-generating NADPH oxidase NOX4 is a critical mediator in oncogenic H-Ras-induced DNA damage and subsequent senescence.

Weyemi U, Lagente-Chevallier O, Boufraqech M, Prenois F, Courtin F, Caillou B, Talbot M, Dardalhon M, Al Ghuzlan A, Bidart JM, Schlumberger M, Dupuy C
Oncogene 2012 Mar 1;31(9):1117-29

(Pro)renin promotes fibrosis gene expression in HEK cells through a Nox4-dependent mechanism.

Clavreul N, Sansilvestri-Morel P, Magard D, Verbeuren TJ, Rupin A
American journal of physiology. Renal physiology 2011 Jun;300(6):F1310-8

High-fat diet induces an initial adaptation of mitochondrial bioenergetics in the kidney despite evident oxidative stress and mitochondrial ROS production.

Ruggiero C, Ehrenshaft M, Cleland E, Stadler K
American journal of physiology. Endocrinology and metabolism 2011 Jun;300(6):E1047-58

Cognitive impairment in humanized APP×PS1 mice is linked to Aβ(1-42) and NOX activation.

Bruce-Keller AJ, Gupta S, Knight AG, Beckett TL, McMullen JM, Davis PR, Murphy MP, Van Eldik LJ, St Clair D, Keller JN
Neurobiology of disease 2011 Dec;44(3):317-26

Glucagon-like peptide-1 receptor agonist ameliorates renal injury through its anti-inflammatory action without lowering blood glucose level in a rat model of type 1 diabetes.

Kodera R, Shikata K, Kataoka HU, Takatsuka T, Miyamoto S, Sasaki M, Kajitani N, Nishishita S, Sarai K, Hirota D, Sato C, Ogawa D, Makino H
Diabetologia 2011 Apr;54(4):965-78

The NADPH oxidase Nox4 restricts the replicative lifespan of human endothelial cells.

Lener B, Kozieł R, Pircher H, Hütter E, Greussing R, Herndler-Brandstetter D, Hermann M, Unterluggauer H, Jansen-Dürr P
The Biochemical journal 2009 Oct 12;423(3):363-74

Mechanisms of podocyte injury in diabetes: role of cytochrome P450 and NADPH oxidases.

Eid AA, Gorin Y, Fagg BM, Maalouf R, Barnes JL, Block K, Abboud HE
Diabetes 2009 May;58(5):1201-11

Increased superoxide formation induced by irradiation preconditioning triggers kidney resistance to ischemia-reperfusion injury in mice.

Kim J, Park JW, Park KM
American journal of physiology. Renal physiology 2009 May;296(5):F1202-11

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nox4 Antibody [NB110-58849] - Detection of NOX4 in HUVEC whole cell lysate. Lanes 1 and 2: serum-starved HUVEC lysate denatured at 95C for 5 minutes. Lanes 3: serum-starved HUVEC lysate denatured at room temperature for 10 minutes. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nox4 Antibody [NB110-58849] - Detection of NOX4 on IMR90 lysate. Image courtesy of Naomi Logsdon, University of Alabama at Birmingham.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Nox4 Antibody [NB110-58849] - Detection of NOX4 in human kidney lysates using NB110-58849 at 2.0 ug/mL. A non-specific band is often observed running at 50 kDa in tissue lysates which is believed to correspond to the human IgG heavy chain.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Nox4 Antibody [NB110-58849] - Detection of NOX4 in proximal convoluted tubules of the kidney using NB110-58849 at 5 ug/mL.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Nox4 Antibody [NB110-58849] - AT1-KO mice are resistant to alcohol-induced oxidative stress. Oxidative stress was examined by immunohistochemical staining of NOX4 (D) expression was examined by immunohistochemical staining. Data are presented as means +/- SD. *, P < 0.05 vs corresponding control; #, P < 0.05 vs WT alcohol group. Bar = 50 uM. Image collected and cropped by CiteAb from the following publication (http://doi.wiley.com/10.1111/j.1582-4934.2012.01569.x), licensed under a CC-BY licence.