14347-1-AP

antibody from Invitrogen Antibodies

Targeting: NOX4 KOX, KOX-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14347-1-AP

Provider

Invitrogen Antibodies

Product name

NOX4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: PGCISLNRT SSQNISLPEY FSEHFHEPFP EGFSKPAEFT QHKFVKICME EPRFQANFPQ TWLWISGPLC LYCAERLYRY IRSNKPVTII SVISHPSDVM EIRMVKENFK ARPGQYITLH CPSVSALENH PFTLTMCPTE TKATFGVHLK IVGDWTERFR DLLLPPSSQD SEILPFIQSR NYPKLYIDGP FGSPFEESLN YEVSLCVAGG IGVTPFASIL NTLLDDWKPY KLRRLYFIWV CRDIQSFRWF ADLLCMLHNK FWQENRPDYV NIQLYLSQTD GIQKIIGEKY HALNSRLFIG RPRWKLLFDE IAKYNRGKTV GVFCCGPNSL SKTLHKLSNQ NNSYGTRFEY NKESFS (224-578 aa encoded by BC040105)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14347-1-AP (NOX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14347-1-AP (NOX4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14347-1-AP (NOX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HUVEC cells were subjected to SDS PAGE followed by western blot with 14347-1-AP (NOX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of BxPC-3 cells using 14347-1-AP (NOX4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 14347-1-AP (NOX4 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 14347-1-AP (NOX4 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14347-1-AP (NOX4 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 14347-1-AP ( NOX4 Antibody) at dilution of 1:200 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were intracellularly stained with 0.2 µg Anti-Human NOX4 (Product # 14347-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.2 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2ug NOX4 antibody (14347-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NOX4 (IP:14347-1-AP, 3ug; Detection:14347-1-AP 1:1000) with HEK-293 cells lysate 1700ug.