PAB21824

antibody from Abnova Corporation

Targeting: RBM28 FLJ10377

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21824

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21824, RRID:AB_10968531

Product name

RBM28 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant RBM28.

Antigen sequence

EHSKGCAFAQFMTQEAAQKCLLAASPENEAGGLKL
DGRQLKVDLAVTRDEAAKLQTTKVKKPTGTRNLYL
AREGLIRAGTKAAEGVSAADMAKRERFELLKHQKL
KDQNIFVSRTR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with RBM28 polyclonal antibody (Cat # PAB21824) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with RBM28 polyclonal antibody (Cat # PAB21824) at 1-4 ug/mL dilution shows positivity in nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human hippocampus with RBM28 polyclonal antibody (Cat # PAB21824) shows distinct nucleolar positivity in neuronal cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)