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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-27792 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IMPDH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 1 mg/mL
Submitted references Target identification reveals protein arginine methyltransferase 1 is a potential target of phenyl vinyl sulfone and its derivatives.
Yu CH, Chou CC, Lee DY, Khoo KH, Chang GD
Bioscience reports 2018 Apr 26;38(2)
Bioscience reports 2018 Apr 26;38(2)
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IMPDH1 Polyclonal Antibody (Product # PA5-27792). Sample (30 µg of whole cell lysate). Lane A: Raji . 7.5% SDS PAGE. IMPDH1 Polyclonal Antibody (Product # PA5-27792) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Confirmation of PVS and PVSN tagging by immunoprecipitation followed by immunoblotting HeLa cells were treated with 500 muM PVS or PVSN in PBS for 5 min and the lysate was subjected to anti-PVS immunoprecipitation. The immunoprecipitate was then probed with anti-protein arginine methyltransferase 1 (PRMT1), anti-glutathione reductase (GR), and anti-vimentin (upper panel). For inosine-5'-monophosphate dehydrogenase 1 (IMPDH1), the lysate was immunoprecipitated with anti-IMPDH1 and then the immunoprecipitate was probed with anti-PVS (lower panel). The control groups were treated with the same volume of DMSO.
