PA1-941

antibody from Invitrogen Antibodies

Targeting: KCNQ5 Kv7.5

Western blot (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-941

Provider

Invitrogen Antibodies

Product name

KCNQ5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-941 detects the KCNQ5 from rat and human samples. This antibody is specific for KCNQ5 and does not detect KCNQ1, KCNQ2, KCNQ3 or KCNQ4. PA1-941 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~125 kDa band representing KCNQ5 from rat brain extract. The PA1-941 immunizing peptide corresponds to amino acid residues 880-897 from human KCNQ5.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Peripheral K(V)7 channels regulate visceral sensory function in mouse and human colon.

Peiris M, Hockley JR, Reed DE, Smith ESJ, Bulmer DC, Blackshaw LA
Molecular pain 2017 Jan-Dec;13:1744806917709371

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Distribution of voltage-gated potassium and hyperpolarization-activated channels in sensory afferent fibers in the rat carotid body.

Buniel M, Glazebrook PA, Ramirez-Navarro A, Kunze DL
The Journal of comparative neurology 2008 Oct 1;510(4):367-77

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The KCNQ/M-current modulates arterial baroreceptor function at the sensory terminal in rats.

Wladyka CL, Feng B, Glazebrook PA, Schild JH, Kunze DL
The Journal of physiology 2008 Feb 1;586(3):795-802

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. Expression of K V 7 channels in mouse colon and mesenteric neurovasculature. (a) K V 7.3 co-expression with CGRP-positive neurons within the myenteric plexus (MP) of mouse colon (tissue sections). Panel (iv) is a schematic representation of endings that co-label for CGRP and K V 7.3 (in black) surrounding neuronal cell bodies (in grey) of the myenteric plexus. (b) In the mesentery associated with the colon, K V 7.3 co-localizes with CGRP-positive neurons (white arrowheads) surrounding blood vessels (BV) in whole mounts. (c) K V 7.5 is expressed in the serosal layer of mouse colon and co-localizes with CGRP-positive fibres in the myenteric plexus (tissue sections). (d) CGRP positive neurons that run alongside blood vessels found within the mesenteric attachment to the colon co-express K V 7.5 (whole mounts). Punctate staining for K V 7.5 is also found around CGRP positive nerve bundle (NF). All images taken at 40X, scale bar = 25 um. Figures b(iii) and d(iii) are 4X of the original images, b(i) and d(i). LM: longitudinal muscle; CM: circular muscle.