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Western blot
Blocking/Neutralizing
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- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- PA5-47087 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL18RAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL-18 R beta (AcPL), recombinant human (rh) IL-18 R, rhIL-1 R1, rhIL-1 RII, and rhIL-1 Rrp2 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Neutralization of IL18RAP in KG‚1 human acute myelogenous leukemia cell line. Samples were incubated in IL18RAP polyclonal antibody (Product # PA5-47087). In the presence of Recombinant Human TNF‚α (20 ng/mL), Recombinant Human IL‚18/IL‚1F4 stimulates IFN‚γ secretion in the KG‚1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN‚γ Quantikine ELISA Kit. Under these conditions, IFN‚γ secretion elicited by Recombinant Human IL‚18/IL‚1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‚18 Rβ/IL‚1 R7 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.3-1.2 µg/mL.
