HPA027776

antibody from Atlas Antibodies

Targeting: PNPO PDXPO

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA027776

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA027776, RRID:AB_10601246

Product name

Anti-PNPO

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

MTCWLRGVTATFGRPAEWPGYLSHLCGRSAAMDLG
PMRKSYRGDREAFEETHLTSLDPVKQFAAWFEEAV
QCPDIGEANAMCLATCTR


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PNPO antibody. Remaining relative intensity is presented.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-PNPO antibody HPA027776 (A) shows similar pattern to independent antibody HPA023204 (B).

Antibody #2 product nr

HPA023204

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human colon, kidney, liver and lymph node using Anti-PNPO antibody HPA027776 (A) shows similar protein distribution across tissues to independent antibody HPA023204 (B).

Antibody #2 product nr

HPA023204

Antibody provider

Atlas Antibodies

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong positivity in cells in tubules.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node using Anti-PNPO antibody HPA027776.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver using Anti-PNPO antibody HPA027776.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney using Anti-PNPO antibody HPA027776.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-PNPO antibody HPA027776.

Sample type

HUMAN