15552-1-AP

antibody from Invitrogen Antibodies

Targeting: PNPO PDXPO

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15552-1-AP

Provider

Invitrogen Antibodies

Product name

PNPO Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MTCWLRGVT ATFGRPAEWP GYLSHLCGRS AAMDLGPMRK SYRGDREAFE ETHLTSLDPV KQFAAWFEEA VQCPDIGEAN AMCLATCTRD GKPSARMLLL KGFGKDGFRF FTNFESRKGK ELDSNPFASL VFYWEPLNRQ VRVEGPVKKL PEEEAECYFH SRPKSSQIGA VVSHQSSVIP DREYLRKKNE ELEQLYQDQE VPKPKSWGGY VLYPQVMEFW QGQTNRLHDR IVFRRGLPTG DSPLGPMTHR GEEDWLYERL AP (1-261 aa encoded by BC006525)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.55 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human heart tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 15552-1-AP (PNPO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using PNPO antibody 15552-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using PNPO antibody 15552-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 15552-1-AP (PNPO antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 15552-1-AP (PNPO antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PNPO (IP:15552-1-AP, 4ug; Detection:15552-1-AP 1:500) with MCF-7 cells lysate 3200 ug.