PA5-27652

antibody from Invitrogen Antibodies

Targeting: TSFM EF-TS, EF-Tsmt

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27652

Provider

Invitrogen Antibodies

Product name

TSFM Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Jurkat, Raji, K562.

Concentration

1 mg/mL

References

Submitted references

Novel compound mutations in the mitochondrial translation elongation factor (TSFM) gene cause severe cardiomyopathy with myocardial fibro-adipose replacement.

Perli E, Pisano A, Glasgow RIC, Carbo M, Hardy SA, Falkous G, He L, Cerbelli B, Pignataro MG, Zacara E, Re F, Della Monica PL, Morea V, Bonnen PE, Taylor RW, d'Amati G, Giordano C
Scientific reports 2019 Mar 25;9(1):5108

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using TSFM Polyclonal Antibody (Product # PA5-27652). Sample (30 µg of whole cell lysate). Lane A: Jurkat. Lane B: K562. 10% SDS PAGE. TSFM Polyclonal Antibody (Product # PA5-27652) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TSFM Polyclonal Antibody detects TSFM protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: TSFM protein stained by TSFM Polyclonal Antibody (Product # PA5-27652) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using TSFM (Product # PA5-27652) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.