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LearnMA3-075
antibody from Invitrogen Antibodies
Targeting: TAF4
TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MA3-075 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAF4 Monoclonal Antibody (20TA-1B12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA3-075 detects TATA-Binding Protein (TBP) Associated factor 4 from human and mouse samples.
- Antibody clone number
- 20TA-1B12
- Concentration
- Conc. Not Determined
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TAF4 was performed by loading 20 µg of HeLa, T47D nuclear lysates and 10-20 µg of C2C12 and F9 whole cell lysates, and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. TAF4 was detected at 135 kDa using a TAF4 mouse monoclonal antibody (Product # MA3-075) at a dilution of 1:1000 in blocking buffer for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:1000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TAF4 in HeLa cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a TAF4 mouse monoclonal antibody (Product # MA3-075) at a dilution of 1:4000 in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:1000 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
