Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00010450-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010450-M02, RRID:AB_547232
- Product name
- Mouse Monoclonal Cyclophilin-E Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PPIE - peptidylprolyl isomerase E (cyclophilin E)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02] - Analysis of PPIE expression in transfected 293T cell line by PPIE monoclonal antibody (M02), clone 2F5.Lane 1: PPIE transfected lysate(33.4 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclophilin-E Antibody (2F5) [H00010450-M02] - PPIE monoclonal antibody (M02), clone 2F5. Analysis of PPIE expression in PC-12.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Cyclophilin-E Antibody (2F5) [H00010450-M02] - Detection limit for recombinant GST tagged PPIE is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Cyclophilin-E Antibody (2F5) [H00010450-M02] - Analysis of monoclonal antibody to PPIE on HeLa cell. Antibody concentration 10 ug/ml.