Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunocytochemistry [3]
- Other assay [1]
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- Product number
- 14-1206-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-20RB Monoclonal Antibody (20RNTC), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 20RNTC monoclonal antibody recognizes mouse and human IL-20R2 (interleukin-20 receptor 2, IL-20Rb) in either of its two heterodimeric complexes formed by pairing with IL-20R1 or IL-22R1. IL-20R2 belongs to the class II cytokine receptor family that shares a characteristic pattern of cysteine, proline and tryptophan residues in the extracellular domain. IL-20R2 paired with IL-20R1 forms a receptor that binds IL-20, IL-19, or IL-24. IL-20R2 paired with IL22R1 forms a receptor that binds IL-20 or IL-24. The IL-20R complex signals primarily via the JAK-STAT pathway. IL-20R2 is found predominantly on cells of epithelial origin such as gut and skin and can be upregulated during inflammation. Immunofluorescent staining of A375 cells reveals a punctate staining pattern at the cell membrane, as well as diffuse, cytoplasmic staining that is likely associated with protein synthesis and transport in the endoplasmic reticulum and golgi. Applications Reported: This 20RNTC antibody has been reported for use in immunocytochemistry, immunoblotting (WB) and flow cytometric analysis. Applications Tested: This 20RNTC antibody has been tested by immunofluorescent staining and flow cytometric analysis of fixed and permeabilized A375 cells. This can be used between 1-10 µg/mL. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 20RNTC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references IL-20 antagonist suppresses PD-L1 expression and prolongs survival in pancreatic cancer models.
Production of Interleukin-20 cytokines limits bacterial clearance and lung inflammation during infection by Streptococcus pneumoniae.
IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas.
Interleukin-19 acts as a negative autocrine regulator of activated microglia.
Signaling via the IL-20 receptor inhibits cutaneous production of IL-1β and IL-17A to promote infection with methicillin-resistant Staphylococcus aureus.
Interleukin (IL)-19, IL-20 and IL-24 are produced by and act on keratinocytes and are distinct from classical ILs.
Lu SW, Pan HC, Hsu YH, Chang KC, Wu LW, Chen WY, Chang MS
Nature communications 2020 Sep 14;11(1):4611
Nature communications 2020 Sep 14;11(1):4611
Production of Interleukin-20 cytokines limits bacterial clearance and lung inflammation during infection by Streptococcus pneumoniae.
Madouri F, Barada O, Kervoaze G, Trottein F, Pichavant M, Gosset P
EBioMedicine 2018 Nov;37:417-427
EBioMedicine 2018 Nov;37:417-427
IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas.
Ross BX, Gao N, Cui X, Standiford TJ, Xu J, Yu FX
Journal of immunology (Baltimore, Md. : 1950) 2017 May 1;198(9):3536-3547
Journal of immunology (Baltimore, Md. : 1950) 2017 May 1;198(9):3536-3547
Interleukin-19 acts as a negative autocrine regulator of activated microglia.
Horiuchi H, Parajuli B, Wang Y, Azuma YT, Mizuno T, Takeuchi H, Suzumura A
PloS one 2015;10(3):e0118640
PloS one 2015;10(3):e0118640
Signaling via the IL-20 receptor inhibits cutaneous production of IL-1β and IL-17A to promote infection with methicillin-resistant Staphylococcus aureus.
Myles IA, Fontecilla NM, Valdez PA, Vithayathil PJ, Naik S, Belkaid Y, Ouyang W, Datta SK
Nature immunology 2013 Aug;14(8):804-11
Nature immunology 2013 Aug;14(8):804-11
Interleukin (IL)-19, IL-20 and IL-24 are produced by and act on keratinocytes and are distinct from classical ILs.
Kunz S, Wolk K, Witte E, Witte K, Doecke WD, Volk HD, Sterry W, Asadullah K, Sabat R
Experimental dermatology 2006 Dec;15(12):991-1004
Experimental dermatology 2006 Dec;15(12):991-1004
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent microscopy of fixed and permeabilized A375 cells using 10 µg/mL of Anti-Human/Mouse IL-20 Receptor 2 (IL-20Rb) Purified (right) or Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (left) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and then Streptavidin FITC (Product # 11-4317-87).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent microscopy of fixed and permeabilized A375 cells using 10 µg/mL of Anti-Human/Mouse IL-20 Receptor 2 (IL-20Rb) Purified (right) or Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (left) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and then Streptavidin FITC (Product # 11-4317-87).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent microscopy of fixed and permeabilized A375 cells using 10 µg/mL of Anti-Human/Mouse IL-20 Receptor 2 (IL-20Rb) Purified (right) or Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (left) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and then Streptavidin FITC (Product # 11-4317-87).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL