- MA5-19117 - Invitrogen Antibodies HOXB9 antibody

Submitted references Insights into homeobox B9: a propeller for metastasis in dormant prostate cancer progenitor cells.

Sui Y, Hu W, Zhang W, Li D, Zhu H, You Q, Zhu R, Yi Q, Tang T, Gao L, Zhu S, Yang T
British journal of cancer 2021 Sep;125(7):1003-1015

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot analysis of HOXB9 expressed in transfected 293T cell line by anti-HOXB9 monoclonal antibody (Product # MA5-19117), clone 2E8. Lane 1: HOXB9 transfected lysate (28.1 KDa). Lane 2: Non-transfected lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Detection limit for recombinant GST tagged HOXB9 using anti-HOXB9 monoclonal antibody (Product # MA5-19117) is 1 ng/mL as a capture antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of HOXB9 transfected lysate using anti-HOXB9 monoclonal antibody (Product # MA5-19117) and Protein A Magnetic Beads, and immunoblotted with HOXB9 rabbit polyclonal antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 The ALDH + CD44 + CXCR4 + CD24 + subpopulation in human PCa tissues. a Western blot analysis was performed to determine the protein expressions of PSA, HOXB9, ALDH, CD44, CXCR4 and CD24 in the controls (PCa tissue with Gleason score 6), para-carcinoma (2 mm away from PCa tissue), initial PCa tissue (derived from PCa at first diagnosis via radical prostatectomy) and refractory PCa tissue (derived after recurrence), respectively. beta-actin was used as an internal control. b Quantification of ( a ). * P < 0.05 vs. initial PCa tissues. ( n = 6). c Human PCa tissue was subcutaneously implanted into NOD-SCID mice to establish a patient-derived xenograft (PDX) model. Subsets of cells (as indicated) were derived from the PDX model and seeded in 96-well plates (1 x 10 4 cells/well) and treated with different anti-androgens (as indicated) and chemotherapeutic agents, with 0.2% DMSO and 0.5% H 2 O 2 were used as negative and positive controls, respectively. After 48 h of treatment, cells were incubated with alamarBlue solution for 4 h, and cell viability was measured with excitation wavelength at 530-560 nm and emission wavelength at 590 nm using a TECAN Infinite 200 PRO microplate reader. d Subsets of cells (as indicated) were derived from the PDX model and seeded in 96-well plates (1 x 10 4 cells/well). Cells were treated with different chemotherapeutic drugs, as indicated, for 72 h. Then, a WST-1 proliferation assay was performed. The absorbance was measured at 450 nm using a

MA5-19117

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